Abstract
In this study, a silver doped mesoporous silica nanoparticles-based enzyme-less electrochemical sensor for the determination of hydrogen peroxide (H2O2) released from live cells was constructed for the first time. The presented electrochemical sensor exhibited fast response (2 s) towards the reduction of H2O2 concentration variation at an optimized potential of −0.5 V with high selectivity over biological interferents such as uric acid, ascorbic acid, and glucose. In addition, a wide linear range (4 μM to 10 mM) with a low detection limit (LOD) of 3 μM was obtained. Furthermore, the Ag-mSiO2 nanoparticles/glass carbon electrode (Ag-mSiO2 NPs/GCE) based enzyme-less sensor showed good electrocatalytic performance, as well as good reproducibility, and long-term stability, which provided a successful way to in situ determine H2O2 released from live cells. It may also be promising to monitor the effect of reactive oxygen species (ROS) production in bacteria against oxidants and antibiotics.
Highlights
The rapid, specific, and accurate hydrogen peroxide (H2O2) determination is quite essential in bioanalytical fields
H2O2, as the most common representative of reactive oxygen species, which endogenously produced in a cell, is a key player in various pathological processes
The determination of H2O2 attracted much interest as its potential as an indicator of oxidative stress-related diseases. Several analytical methods such as spectrophotometry [3], fluorimetry [4], titrimetry [5], chromatography [6], and electrochemistry [7,8] have been applied for highly sensitive H2O2 determination
Summary
The rapid, specific, and accurate hydrogen peroxide (H2O2) determination is quite essential in bioanalytical fields. The determination of H2O2 attracted much interest as its potential as an indicator of oxidative stress-related diseases. Several analytical methods such as spectrophotometry [3], fluorimetry [4], titrimetry [5], chromatography [6], and electrochemistry [7,8] have been applied for highly sensitive H2O2 determination. Most electrode modifiers possessed high potentials, which limited the sensitive and selective. HSigilhvecrosnta, nsuopscaerptitcilbelse (cAongfoNrPmsa)tieoxnhiabffitecgtoeoddbysypnHer,gtemticpefrfaetcutreo,netHc.2[O125]r.edSiulvcteironna[n1o6p,1a7r]t,icwlehsic(Ah gis NwPisd)eelyxhuibsietdgofoodr seynnzeyrmgeet-ilceessffeeclteoctnroHc2hOem riceadlucsteinosno[r1s6,o1f7]H, w2Oh2i.chHisoweidvelry, uAsgedNfoPrsenarzeymeaes-lyestso ealegcgtroegchaetemiincaslosleuntsiorns boef fHor2eOm2. [a1c0h]iecovue lad laocwhieervLeOaDlowofe0r.L45OμDMof. W0.4h5ilμeMth.eWshigilneiftihceanstigsnuicficceasnsthsausccbeesesn hmasabdeeninmtahdeedinettehcetidoneteocftiotrnacoefstroafceHs o2Of 2H, 2wOh2i,cwhhiischsuismsummarmizaerdizeind itnhethenenwewwwoorkrkbbyy Viitteerr aanndd IaIatstsuunnsskkyyii[2[255],],bbioiosesennssoorsrsbbaaseseddoonnAAgg-d-dooppededMMCCMM-4-141mmSiSOiO2 2NNPPssfofor rreraela-lt-itmimeeddeteetcetcitoinonofoHf H2O2O2 2 rereleleaasseeddfrforommlilviveecceelllslshhaassnnoot tbbeeennrerpepoortretdedyyete,tt,otoththeebbesetstoof fouour rkknnowowleldedgeg.e. WWeepprerseesnenteteddananenenzyzymme-el-elsesseleelcetcrtorochcheemmiciacal lbbioiosesennsosorrbbaaseseddoonnAAgg-d-dooppededMMCCMM-4-411mmSSiOiO2 2 NNPPsstotoddeetetermrminineeHH2 O2O22 rreelleeaasseedd ffrroommlliivveecceellsls((FFigiguurree11).).OOuurrrreessuultltsshhoowweeddaahhigighheelelecctrtoroccaatatalylytitcic ppeerfroformrmaanncceeoof fAAgg-m-mSSiOiO2 2nnaannooppaarrtticicleless/g/glalassssccaarrbboonneelelecctrtrooddee(N(NPPss/G/GCCEE) )totowwaardrdssHH2 O2O22 rreedduuccttiioonn. AAdddditiitoionnalallyl,ya, gaogoodolidnelianreraarngraenfrgoemfr4oμmM4toμ1M0 mtoM1w0 imthMa LwOiDthoaf 3LμOMDwoaf s3obμtMainweda.s Tohbetapirneesden. tTehde spenressoernstheodwsednshoirghshsoewleectdivhitigyhovselrebctioivloitgyicoavleirntbeirofleorgenictasl, isnutcehrfaesreansctso,rsbuicchacaids ,auscriocrbacicida,caidnd, ugrliuccaocsied., Manodreogvluecr,oHse.OM2 orreeleoavseerd, Hfro2Om2 prehleeoacshedrofmroomcyptohmeoac(hPrCo-m12o)ccyetollms cao(uPldC-b1e2)succecllessscfouulllyd dbeetescutcecdesussfiunlgly thdeetpercetseedntusseinnsgort.hIenpardedsietniotns,eanssroera.ctIinveaodxdyitgieonn,spaescireesa(cRtiOveS)oinxyclguedninsgpHec2iOes (pRrOodSu)citniocnluidnibnagctHer2iOa2 spurcohdauscEtioscnheirnichbiaaccteolriiaansducVhagaisnaEl sLcahcetroicbhaicaillciocliananindcrVeaagsienathl eLabcatcotbearciiall’is csaunscienpctriebailsietytthoeobxaidctaetriivae’s astutascckepfrtoibmiliatnytitboiootxicidtaretiavtme aetntta[c2k6,f2r7o]m, oaunrtsibeniostoirc itsrepartommeinsitn[g26to,2h7e],lpouurndseenrsstoarndisinpgrothmeisminegchtaonhisemlp ouf nHd2eOrs2tapnroddinugcitnhge bmyebcahcatneirsima aogfaHin2sOt 2anptriobdioutcicinsginbay cblaincitce.ria against antibiotics in a clinic
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