Abstract
A solid phase microextraction fiber based on high temperature silicone glue coated on a stainless steel wire is presented for use in the extraction of amino acid derivatives. Amino acids were derivatizad using ethyl chloroformate. Effects of the extraction and desorption parameters including extraction and desorption time, desorption temperature and ionic strength on the extraction/desorption efficiency have been studied and quality parameters of the method were measured. A possibility to apply the proposed method for the identification of proteinaceous binders was demonstrated.
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