Abstract
This study aimed to explore the effects of nucleate agent sizes on lysozyme crystallization. Silica nanoparticles (SNP) with four different particle sizes of 5 nm, 15 nm, 50 nm, and 100 nm were chosen for investigation. Studies were carried out both microscopically and macroscopically. After adding SNP, the morphological defects of lysozyme crystals decreased, and the number of crystals increases with the size of the SNP. The interaction between SNP and lysozyme was further explored using UV spectroscopy, fluorescence spectroscopy, and Zeta potential. It was found that the interaction between SNP and lysozyme was mainly electrostatic interaction, which increased with the size of SNP. As a result, lysozyme could be attracted to the surface of SNP and aggregated to form the nucleus. Finally, the activity test and circular dichroism showed that SNP had little effect on protein secondary structure.
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