Abstract
BackgroundBone morphogenetic protein 2 (BMP-2) is a member of the TGF-β superfamily that is closely correlated with many malignancies, particularly lung cancer. However, the effects of silenced BMP-2 on lung cancer cell proliferation and migration are not clear.MethodsUsing quantitative real-time RT-PCR, BMP-2 mRNA expression was detected in 61 non-small cell lung cancer (NSCLC) samples. Survival curves were generated using follow-up data. Relationships between clinical or pathological characteristics and prognosis were analyzed. Cell viability assays and transwell migration assays were used to evaluate the effects of BMP-2 silencing on cell proliferation and migration of A549 and H460 cells.ResultsBMP-2 mRNA expression was higher in NSCLC tissues compared to matched adjacent normal tissues (P < 0.01). High BMP-2 expression levels were significantly associated with the occurrence of lymph node metastases and tumor stage (P < 0.05). There were significant differences in survival curves between groups with metastatic lymph nodes and non-metastatic lymph nodes, as well as between groups with low BMP-2 expression and groups with high BMP-2 expression. In addition, we observed decreased proliferation and migration rates of the NSCLC-derived cell lines A549 and H460 that were transfected with siBMP-2 (P < 0.05).ConclusionBMP-2 mRNA is overexpressed in NSCLC samples and is a risk factor for survival in patients with NSCLC. BMP-2 silencing can significantly inhibit A549 and H460 cell proliferation and migration.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4263254471298866
Highlights
Bone morphogenetic protein 2 (BMP-2) is a member of the TGF-β superfamily that is closely correlated with many malignancies, lung cancer
Bone morphogenetic proteins (BMPs)-2 mRNA is overexpressed in non-small-cell lung cancer (NSCLC) samples To explore the relationship between BMP-2 mRNA expression and NSCLC clinicopathological characteristics, we retrospectively analyzed 61 NSCLC patients
We evaluated the expression of BMP-2 mRNA using quantitative real time RT-PCR, and explored the relationship between BMP-2 mRNA levels and TNM stage, node status, gender, age, differentiation, and tumor stage
Summary
Bone morphogenetic protein 2 (BMP-2) is a member of the TGF-β superfamily that is closely correlated with many malignancies, lung cancer. The effects of silenced BMP-2 on lung cancer cell proliferation and migration are not clear. 80% of diagnosed lung cancer cases are non-small-cell lung cancer (NSCLC) [4,5]. Bone morphogenetic protein 2 (BMP-2) was originally identified as an osteoinductive cytokine, and was subsequently reported to have an important role in cell migration, proliferation, and differentiation [6,7]. We used siRNA to silence BMP-2 to observe the effect on proliferation and migration of the lung cancer cell lines A549 and H460. We analyzed the correlation between BMP-2 mRNA expression and the clinicopathological characteristics of 61 patients with NSCLC
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