Abstract
Full Text Article Outline I. Objective II. Methods III. Results IV. Conclusion Objective The cardiomyocytes of failing hearts show a significant reduced ability to synthesize ATP. On the one hand, the ATP synthesis depends on the level of the H+ based mitochondrial membrane potential which is impaired in heart failure and which in turn correlates to the electron transfer rate of the respiratory chain of the mitochondria. On the other hand, the electron transfer rate can be changed by external electric fields. We thought to examine the effect of electric fields concomitant with low and high electric microcurrent on the capability of mitochondria of cultured cardiomyocytes from spontaneous hypertensive rates to synthesize ATP. Methods Cardiomyocytes of the myocardium of spontaneous hypertensive rats (N=5; 11 weeks old) were stimulated electrically by use of a direct current (dc) power generator via two electrodes under cultured conditions. Of each SHR myocardium, five times three specimens of myocytes were taken, cultured and exposed to the dc with the intensity zero (control), 10 (low) or 100 (high) μA over a period of 72h. Mitochondrial respiration of dc treated cells was measured via the Oxygraph 2K (Oroboros, Innsbruck, Austria). This instrument allows the continuous measurement of oxygen consumption of intact cells. A sequence of the inhibitors oligomycin, carbonyl cyanide4-(trifluoromethoxy)phenylhydrazone and rotenone was added to analyze the impact of different mitochondrial complexes on respiration. ATP of microcurrent treated SHR cardiomyocytes was isolated by an ATP-assay (Abcam, ab83355) and quantified fluorometrically. Article Tools PDF (226 KB) Email Article Add to My Reading List Export Citation Create Citation Alert Cited by in Scopus (0) Request Permissions Order Reprints (100 minimum order) Related Articles All Content Search Advanced Search | Register August 2015 Volume 21, Issue 8, Supplement, Page S19 Karin Macfelda Alexander Holly Johannes Mueller Access this article on ScienceDirect Home Articles & Issues For Authors Journal Info Subscribe Society More Periodicals
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