Abstract
Ki-Won Lee, Ochirbat Chinzorig, Gi Jun Choi, Sei Hyung Yoon, Ki-Yong Kim,Hee Chung Ji and Sang-Hoon LeeABSTRACTSuccess in molecular breeding for better adapted varieties to environmental stresses depend upon the concerted efforts by various research including tissue culture, transformation, genetics and breeding. In order to optimize tissue culture conditions of Siberian wildrye grass, the effects of plant growth regulators on callus induction and plant regeneration were investigated with mature seeds. The highest callus induction frequency was observed when the mature seeds were cultured on MS medium supplemented with 5mg/L 2,4-D. The highest plant regeneration frequency was observed when callus was transferred to N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Regenerated plants were grown normally when shoots were transplanted to the soil. A short tissue culture period and regeneration system would be beneficial for molecular breeding of Siberian wildrye grass by the production of transgenic plant.(Key words:Siberian wildrye grass, Callus, Plant regeneration, Plant growth regulators)
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