Short electromyographic bursts in the rabbit digastric muscle during the jaw-closing phase

Abstract

Masseter and digastric muscle activities and jaw movement trajectories were recorded in freely moving rabbits during eating. The patterns in these trajectories and activities were similar to those described in previous studies on restrained animals. Although the duration of masticatory sequences, which started with food intake followed by grinding movements and ended by swallowing, varied, the total number of chewing cycles in a chain of masticatory sequences was consistent (1043 ± 51, mean ± SD; n = 5, for chow pellets) among the animals tested. When animals ate hard foods, extra bursts in the digastric electromyograms occurred frequently in the jaw-closing phase. The digastric activities were rather short (6.1 ± 1.0 ms; n = 100) and the amplitude of these digastric short bursts (DSBs) was much larger (1.69 ± 0.81 mV; n = 100) than in the opening phase (0.56 ± 0.33 mV; n = 100), which actually depressed the jaw. When a soft food (bread) was tested, this activity was not observed. The proportion of occurrences of the DSB in a chewing cycle was high at the slow-closing phase, indicating that the DSBs were due to tooth contacts during food crushing. Of 1035 chewing cycles examined in the five animals, 124 were associated with a DSB and 415 cycles with a masseter inhibitory period (MIP). The proportion of the occurrences of the MIP was significantly larger than that of the DSBs. Of 124 DSBs, 85 (68.5 per cent) coincided with an MIP. Four were not associated with clear MIPs, although there was masseter activity at the time of the DSBs. The other 35 DSBs were out of phase with the masseter bursts, although still in a closing phase. The durations of the MIPs accompanied by a DSB were significantly longer than those not so associated. The DSB may be a reflex response mediated by periodontal mechanoreceptors when the upper and lower teeth come together while chewing hard food. The reflex arc for the DSB may be independent of that for the MIP, and the threshold for the DSB may be higher.