Abstract
Because residual antibiotics in dried distillers grains with solubles (DDGS) could lead to inadvertent feeding of antibiotics to animals, the objective of our study was to determine if a commercial DDGS contained antibiotics. The DDGS used in a feeding study, and milk from cows fed the DDGS, were below the detection limits for at least 17 antibiotics. Additionally, we evaluated if DDGS had any antimicrobial effect against Salmonella Typhimurium, Listeria innocua, Escherichia coli ATCC 25922, Staphylococcus aureus, Pediococcus acidilactici, Lactobacillus casei, Lactobacillus acidophilus, Bacillus licheniformis, Paenibacillus odorifer, Pseudomonas fluorescens, and Paenibacillus amylolyticus using the disk diffusion seeded agar overlay method. Neither the buffered nor nonbuffered water-soluble fractions of DDGS yielded clear zones around disks, indicating that the water-soluble DDGS fraction had no antimicrobial properties against any of the microorganisms tested. The absence of antibiotic residues in DDGS and milk samples in this study confirmed that this source of DDGS can be used as livestock feed without fear of inadvertent feeding of antibiotics.
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