Abstract
Spanish bee samples were analyzed for the presence of Israel Acute Paralysis Virus (IAPV). Some of these samples were collected from colonies presenting compatible symptoms with the colony collapse disorder (CCD, 240 out of 484) and the rest were asymptomatic. Only one of these samples was diagnosed as positive to IAPV by employing a one step RT-PCR that targets the ORF 2 of the IAPV genome. Specificity of the RT-PCR assay was evaluated by sequence analysis of size specific amplification products. IAPV nucleotide sequences already published in GeneBank were used to construct a phylogenetic tree that included the new Spanish IAPV sequence (FJ821506). They segregated in two main lineages and the Spanish isolate was mainly related with the American ones. As IAPV was detected in Spain in a very low frequency, no causal relation between IAPV and CCD in Spain was found.
Highlights
Spanish bee samples were analyzed for the presence of Israel Acute Paralysis Virus (IAPV)
Periodic and significant losses of honeybee colonies have been reported. These days, such losses are increasing in frequency, magnitude and geographical distribution being described as a wide variety of symptoms gathered into the complex named colony collapse disorder (CCD)
This new pathogen is the Israeli Acute Paralysis Virus (IAPV; Maori et al, 2007), which was classified as a new member of the Dicistroviridae family (Christian et al, 2005)
Summary
Spanish bee samples were analyzed for the presence of Israel Acute Paralysis Virus (IAPV). First detection of Israeli Acute Pralysis Virus (IAPV) in Spanish honeybees The first detection of IAPV in bee samples from Spain is reported.
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