Shiraishi Research Group, Nakazawa Laboratory, Electronics and Mechanical Science Division and Industry-Academia Collaboration Promotion Division, Graduate School of Science and Technology, Gunma University

Soft X-ray quantum efficiency of the CCD camera for the Astro-E

Centromere that plays a pivotal role in chromosome segregation is composed of repetitive elements in many eukaryotes. Although chromosomal regions containing repeats are the hotspots of rearrangements, little is known about the stability of centromere repeats. Here, by using a minichromosome that has a complete set of centromere sequences, we have developed a fission yeast system to detect gross chromosomal rearrangements (GCRs) that occur spontaneously. Southern and comprehensive genome hybridization analyses of rearranged chromosomes show two types of GCRs: translocation between homologous chromosomes and formation of isochromosomes in which a chromosome arm is replaced by a copy of the other. Remarkably, all the examined isochromosomes contain the breakpoint in centromere repeats, showing that isochromosomes are produced by centromere rearrangement. Mutations in the Rad3 checkpoint kinase increase both types of GCRs. In contrast, the deletion of Rad51 recombinase preferentially elevates isochromosome formation. Chromatin immunoprecipitation analysis shows that Rad51 localizes at centromere around S phase. These data suggest that Rad51 suppresses rearrangements of centromere repeats that result in isochromosome formation.

Journal of Mass SpectrometryVolume 50, Issue 3 p. 451-453 JMS letters Quantitative chiral analysis of tryptophan using enantiomer-selective photolysis of cold non-covalent complexes in the gas phase Akimasa Fujihara, Corresponding Author Akimasa Fujihara Department of Chemistry, Graduate School of Science, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531 JapanCorrespondence to: Akimasa Fujihara, Department of Chemistry, Graduate School of Science, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan. E-mail: fujihara@c.s.osakafu-u.ac.jpSearch for more papers by this authorNaoto Maeda, Naoto Maeda Department of Chemistry, Graduate School of Science, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531 JapanSearch for more papers by this authorShigeo Hayakawa, Shigeo Hayakawa Department of Chemistry, Graduate School of Science, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531 JapanSearch for more papers by this author Akimasa Fujihara, Corresponding Author Akimasa Fujihara Department of Chemistry, Graduate School of Science, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531 JapanCorrespondence to: Akimasa Fujihara, Department of Chemistry, Graduate School of Science, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan. E-mail: fujihara@c.s.osakafu-u.ac.jpSearch for more papers by this authorNaoto Maeda, Naoto Maeda Department of Chemistry, Graduate School of Science, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531 JapanSearch for more papers by this authorShigeo Hayakawa, Shigeo Hayakawa Department of Chemistry, Graduate School of Science, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531 JapanSearch for more papers by this author First published: 17 February 2015 https://doi.org/10.1002/jms.3547Citations: 21Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume50, Issue3March 2015Pages 451-453 RelatedInformation

Astronomische NachrichtenVolume 320, Issue 4-5 p. 371-371 Original Paper Measurement of the final charge cloud shape produced by an X-ray photon inside the CCD J. Hiraga, J. Hiraga jhiraga@ess.eci.osaka-u.ac-jp Department of Earth and Space Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560 - 0043, JapanSearch for more papers by this authorH. Tsunemi, H. Tsunemi Department of Earth and Space Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560 - 0043, JapanSearch for more papers by this authorK. Yoshita, K. Yoshita Department of Earth and Space Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560 - 0043, JapanSearch for more papers by this authorE. Miyata, E. Miyata Department of Earth and Space Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560 - 0043, JapanSearch for more papers by this author J. Hiraga, J. Hiraga jhiraga@ess.eci.osaka-u.ac-jp Department of Earth and Space Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560 - 0043, JapanSearch for more papers by this authorH. Tsunemi, H. Tsunemi Department of Earth and Space Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560 - 0043, JapanSearch for more papers by this authorK. Yoshita, K. Yoshita Department of Earth and Space Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560 - 0043, JapanSearch for more papers by this authorE. Miyata, E. Miyata Department of Earth and Space Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560 - 0043, JapanSearch for more papers by this author First published: 03 July 2001 https://doi.org/10.1002/1521-3994(199908)320:4/5<371::AID-ASNA371>3.0.CO;2-WCitations: 1AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article.Citing Literature Volume320, Issue4-5August 1999Pages 371-371 RelatedInformation

Journal of Mass SpectrometryVolume 43, Issue 4 p. 535-537 JMS letter High-energy collision-induced dissociation of phosphopeptides using a multi-turn tandem time-of-flight mass spectrometer ‘MULTUM-TOF/TOF’ Shuichi Shimma, Corresponding Author Shuichi Shimma shuichi@mass.phys.sci.o saka-u.ac.jp Department of Physics, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan These authors equally contributed to this workDepartment of Physics, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan.===Search for more papers by this authorHirofumi Nagao, Hirofumi Nagao Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka Univeristy, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan These authors equally contributed to this workSearch for more papers by this authorAnastassios E. Giannakopulos, Anastassios E. Giannakopulos ThermoFisher Scientific, Hanna-Kunath-Str.11, 28199 Bremen, GermanySearch for more papers by this authorShigeo Hayakawa, Shigeo Hayakawa Department of Chemistry, Graduate School of Science, Osaka Prefecture University, 1-1 Gakuencho, Sakai, Osaka, 599-8531, JapanSearch for more papers by this authorKunio Awazu, Kunio Awazu Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka Univeristy, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanSearch for more papers by this authorMichisato Toyoda, Michisato Toyoda Department of Physics, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, JapanSearch for more papers by this author Shuichi Shimma, Corresponding Author Shuichi Shimma shuichi@mass.phys.sci.o saka-u.ac.jp Department of Physics, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan These authors equally contributed to this workDepartment of Physics, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan.===Search for more papers by this authorHirofumi Nagao, Hirofumi Nagao Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka Univeristy, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan These authors equally contributed to this workSearch for more papers by this authorAnastassios E. Giannakopulos, Anastassios E. Giannakopulos ThermoFisher Scientific, Hanna-Kunath-Str.11, 28199 Bremen, GermanySearch for more papers by this authorShigeo Hayakawa, Shigeo Hayakawa Department of Chemistry, Graduate School of Science, Osaka Prefecture University, 1-1 Gakuencho, Sakai, Osaka, 599-8531, JapanSearch for more papers by this authorKunio Awazu, Kunio Awazu Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka Univeristy, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanSearch for more papers by this authorMichisato Toyoda, Michisato Toyoda Department of Physics, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, JapanSearch for more papers by this author First published: 11 December 2007 https://doi.org/10.1002/jms.1352Citations: 12 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume43, Issue4April 2008Pages 535-537 RelatedInformation

The karyopherin CRM1 mediates nuclear export of proteins and ribonucleoproteins bearing a leucine-rich nuclear export signal (NES). To elucidate the precise mechanism by which NES-cargos are dissociated from CRM1 in the cytoplasm, which is important for transport directionality, we determined a 2.0-A resolution crystal structure of yeast CRM1:RanBP1:RanGTP complex, an intermediate in the disassembly of the CRM1 nuclear export complex. The structure shows that on association of Ran-binding domain (RanBD) of RanBP1 with CRM1:NES-cargo:RanGTP complex, RanBD and the C-terminal acidic tail of Ran induce a large movement of the intra-HEAT9 loop of CRM1. The loop moves to the CRM1 inner surface immediately behind the NES-binding site and causes conformational rearrangements in HEAT repeats 11 and 12 so that the hydrophobic NES-binding cleft on the CRM1 outer surface closes, squeezing out the NES-cargo. This allosteric mechanism accelerates dissociation of NES by over two orders of magnitude. Structure-based mutagenesis indicated that the HEAT9 loop also functions as an allosteric autoinhibitor to stabilize CRM1 in a conformation that is unable to bind NES-cargo in the absence of RanGTP.

[reaction: see text] Bowl-shaped phosphine ligands were found to be highly effective in Suzuki-Miyaura coupling of unactivated aryl chlorides, in which the depth of the bowl affected the catalytic activity considerably.

Astronomische NachrichtenVolume 320, Issue 4-5 p. 344-344 Original Paper A new galactic supernova remnant in the direction of G69.7+1.0 K. Yoshita, K. Yoshita Department of Earth and Space Science, Graduate School of Science, Osaka University, JapanSearch for more papers by this authorE. Miyata, E. Miyata Department of Earth and Space Science, Graduate School of Science, Osaka University, JapanSearch for more papers by this authorH. Tsunemi, H. Tsunemi Department of Earth and Space Science, Graduate School of Science, Osaka University, JapanSearch for more papers by this author K. Yoshita, K. Yoshita Department of Earth and Space Science, Graduate School of Science, Osaka University, JapanSearch for more papers by this authorE. Miyata, E. Miyata Department of Earth and Space Science, Graduate School of Science, Osaka University, JapanSearch for more papers by this authorH. Tsunemi, H. Tsunemi Department of Earth and Space Science, Graduate School of Science, Osaka University, JapanSearch for more papers by this author First published: 03 July 2001 https://doi.org/10.1002/1521-3994(199908)320:4/5<344::AID-ASNA344>3.0.CO;2-WCitations: 1AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article.Citing Literature Volume320, Issue4-5August 1999Pages 344-344 RelatedInformation

Annals of the New York Academy of SciencesVolume 67, Issue 4 p. 57-121 CYTOLOGY AND CYTOCHEMISTRY OF MELANOMA CELLS Sylvia S. Greenberg, Sylvia S. Greenberg Department of Biology, Graduate School of Arts and Science, New York University, Washington Square, New York, N. Y.Search for more papers by this authorM. J. Kopac, M. J. Kopac Department of Biology, Graduate School of Arts and Science, New York University, Washington Square, New York, N. Y.Search for more papers by this authorMyron Gordon, Myron Gordon Department of Biology, Graduate School of Arts and Science, New York University, Washington Square, New York, N. Y.Search for more papers by this author Sylvia S. Greenberg, Sylvia S. Greenberg Department of Biology, Graduate School of Arts and Science, New York University, Washington Square, New York, N. Y.Search for more papers by this authorM. J. Kopac, M. J. Kopac Department of Biology, Graduate School of Arts and Science, New York University, Washington Square, New York, N. Y.Search for more papers by this authorMyron Gordon, Myron Gordon Department of Biology, Graduate School of Arts and Science, New York University, Washington Square, New York, N. Y.Search for more papers by this author First published: October 1956 https://doi.org/10.1111/j.1749-6632.1956.tb46039.xCitations: 33 From a thesis presented to the Faculty of the Graduate School of Arts and Science, New York University, by Sylvia S. Greenberg, in partial fulfillment of the requirements for the degree of Doctor of Philosophy. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume67, Issue4Cytology and Cytochemistry of Melanoma CellsOctober 1956Pages 57-121 RelatedInformation

Astronomische NachrichtenVolume 320, Issue 4-5 p. 370-370 Original Paper Pair processes in accretion disks and the jet formation T. Yamasaki, T. Yamasaki Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, JapanSearch for more papers by this authorF. Takahara, F. Takahara Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, JapanSearch for more papers by this authorM. Kusunose, M. Kusunose Department of Physics, School of Science, Kwansei Gakuin University, Nishinomiya, JapanSearch for more papers by this author T. Yamasaki, T. Yamasaki Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, JapanSearch for more papers by this authorF. Takahara, F. Takahara Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, JapanSearch for more papers by this authorM. Kusunose, M. Kusunose Department of Physics, School of Science, Kwansei Gakuin University, Nishinomiya, JapanSearch for more papers by this author First published: 03 July 2001 https://doi.org/10.1002/1521-3994(199908)320:4/5<370::AID-ASNA370>3.0.CO;2-%23AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume320, Issue4-5August 1999Pages 370-370 RelatedInformation

Event Abstract Back to Event Porous hydrogel of wool hair keratin as a substrate for cell culture Ozaki Yuki1*, Yusuke Takagi1*, Hideki Mori1* and Masayuki Hara1* 1 Osaka Prefecture Univertisy, Department of Biological Science, Graduate School of Science,, Japan Introduction: Keratin is contained rich in animal epidermal tissues including hair, nail, horn, and also common proteins such as wool fibers. Keratins have not been so much utilized as Type I collagen as biomaterials although much amount is available as a bio-resource. In this study, we show a novel simple method to prepare a sponge-like porous keratin hydrogel. Wool keratin was extracted for 18 h at 60 oC in a solution containing 8 M guanidine hydrochloride and 2-mercaptoethanol, followed by dialysis against water. Specific water content (%) was calculated as an index for swelling of the gel according to the following equation. Specific water content = (Ww-Wd)/Wd×100 (%) Wd: dry weight of the gel after lyophilization; Ww: wet weight of the gel after swelling in either PBS(-) or DMEM. We tried cell culture on the gel and evaluated the cell growth by WST assay. All animal experiments were conducted in accordance with institutional guidelines and national standards with approval from the Animal Experiment Committee of Osaka Prefecture University. Results and Discussion: Extracted keratin was aggregated and recrosslinked to form a porous hydrogel like a sponge. Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) proved that Type I and Type II keratin subunits were extracted by that procedure without degradation by hydrolysis. The porous keratin gel had a highly porous structure and a fast-swelling property in rehydration after freeze-drying. The gel could be autoclaved without significant change of mechanical strength and shapes. It had also high mechanical strength both in the tensile test and the measurement of dynamic viscoelasticity. The gel is flexible but mechanically durable especially for tensile strain. Those property was different from that of the chemically crosslinked type I collagen gel. Three types of animal cells, PC12 cells, HOS cells and murine embryonic fibroblasts, well attached and grew on the surface of the porous hydrogel. We observed those cells by fluorescent microscopy after live/dead staining and also by a scanning electron microscope. The keratin hydrogel has properties suitable for scaffold in tissue engineering. We tried some preliminary studies on application of the keratin hydrogel for bone tissue engineering by culturing osteoblasts. Reference: Ozaki, Y; Takagi, Y; Mori, H; Hara, M. Mater. Sci. Eng. C 2014, 42, 146–154. Keywords: Tissue Engineering, stem cell, 3D scaffold, Biodegradable material Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Regenerative medicine: biomaterials for control of tissue induction Citation: Yuki O, Takagi Y, Mori H and Hara M (2016). Porous hydrogel of wool hair keratin as a substrate for cell culture. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01232 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. * Correspondence: Dr. Ozaki Yuki, Osaka Prefecture Univertisy, Department of Biological Science, Graduate School of Science,, Sakai-city, Osaka Prefecture, Japan, yuki.ozaki@cira.kyoto-u.ac.jp Dr. Yusuke Takagi, Osaka Prefecture Univertisy, Department of Biological Science, Graduate School of Science,, Sakai-city, Osaka Prefecture, Japan, st304016@edu.osakafu-u.ac.jp Dr. Hideki Mori, Osaka Prefecture Univertisy, Department of Biological Science, Graduate School of Science,, Sakai-city, Osaka Prefecture, Japan, morihide@b.s.osakafu-u.ac.jp Dr. Masayuki Hara, Osaka Prefecture Univertisy, Department of Biological Science, Graduate School of Science,, Sakai-city, Osaka Prefecture, Japan, hara@b.s.osakafu-u.ac.jp Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ozaki Yuki Yusuke Takagi Hideki Mori Masayuki Hara Google Ozaki Yuki Yusuke Takagi Hideki Mori Masayuki Hara Google Scholar Ozaki Yuki Yusuke Takagi Hideki Mori Masayuki Hara PubMed Ozaki Yuki Yusuke Takagi Hideki Mori Masayuki Hara Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

The Anatomical RecordVolume 93, Issue 4 p. 355-361 Article The distribution of alkaline phosphatase in the cyclic growth of the rat hair follicle P. L. Johnson, P. L. Johnson Departments of Anatomy, College of Dentistry and The Graduate School of Arts and Science, New York UniversitySearch for more papers by this authorE. O. Butcher, E. O. Butcher Departments of Anatomy, College of Dentistry and The Graduate School of Arts and Science, New York UniversitySearch for more papers by this authorG. Bevelander, G. Bevelander Departments of Anatomy, College of Dentistry and The Graduate School of Arts and Science, New York UniversitySearch for more papers by this author P. L. Johnson, P. L. Johnson Departments of Anatomy, College of Dentistry and The Graduate School of Arts and Science, New York UniversitySearch for more papers by this authorE. O. Butcher, E. O. Butcher Departments of Anatomy, College of Dentistry and The Graduate School of Arts and Science, New York UniversitySearch for more papers by this authorG. Bevelander, G. Bevelander Departments of Anatomy, College of Dentistry and The Graduate School of Arts and Science, New York UniversitySearch for more papers by this author First published: December 1945 https://doi.org/10.1002/ar.1090930406Citations: 31AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Citing Literature Volume93, Issue4December 1945Pages 355-361 RelatedInformation

Journal Article Purification of β-Glucan Synthase II from Suspension-cultured Rice Cells Get access Ikuo Kuribayashi, Ikuo Kuribayashi Department of Biosystem Science, Graduate School of Science and Technology, Niigata University, 2-Ikarashi, Niigata 950–21, Japan Search for other works by this author on: Oxford Academic Google Scholar Takuya Morita, Takuya Morita Department of Biosystem Science, Graduate School of Science and Technology, Niigata University, 2-Ikarashi, Niigata 950–21, JapanDepartment of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, 2-Ikarashi, Niigata 950–21, Japan Search for other works by this author on: Oxford Academic Google Scholar Toshiaki Mitsui, Toshiaki Mitsui Department of Biosystem Science, Graduate School of Science and Technology, Niigata University, 2-Ikarashi, Niigata 950–21, JapanDepartment of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, 2-Ikarashi, Niigata 950–21, Japan To whom correspondence should be addressed. Search for other works by this author on: Oxford Academic Google Scholar Ikuo Igaue Ikuo Igaue Department of Biosystem Science, Graduate School of Science and Technology, Niigata University, 2-Ikarashi, Niigata 950–21, JapanDepartment of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, 2-Ikarashi, Niigata 950–21, Japan Search for other works by this author on: Oxford Academic Google Scholar Bioscience, Biotechnology, and Biochemistry, Volume 57, Issue 4, 1 January 1993, Pages 682–684, https://doi.org/10.1271/bbb.57.682 Published: 01 January 1993 Article history Received: 26 October 1992 Published: 01 January 1993

The increased worldwide use of shallow geothermal energy systems including ground source heat pumps (GSHPs) have given concerns of possible temperature effects on soil geotechnical properties. In this study, the effects of temperature on mechanical characteristics such as consolidation settlement, shear stiffness, and permeability of kaolin clay were investigated. A modified oedometer apparatus which allows the simultaneous measurements of consolidation settlement, shear wave velocity, and hydraulic conductivity was developed and used. Consolidation tests on preconsolidated kaolin samples (two sample sizes: ϕ 6 cm x H 10 cm and ϕ 6 cm x H 2 cm) were performed under sequentially increasing consolidation pressures at three different temperatures (5 °C, 15 °C, and 40 °C). Larger apparent preconsolidation pressure, Pac, was seen at higher temperature (40 °C) for both sample sizes, but only for samples having relatively high initial void ratios between 1.53 and 1.62. Relatively higher shear modulus as a function of void ratio was observed for samples at higher temperature, suggesting that changes in fabric structure (likely caused by enhanced inter-particle forces between clay particles at higher temperature) resulted in the increased shear stiffness and, thus, higher Pac at 40 °C. Oppositely, temperature effects on the Manuscript received February 5, 2013. This work was partly funded by a grant from the Research Management Bureau, Saitama University, the grant-in-Aid for Scientific Research of Japan Society for the Promotion of Science (JSPS) (No.22860012), and a JSPS bilateral research project. This work was also partially supported by a CREST project, a research grant from the Japan Science and Technology Agency (JST). E. E. Mon is with Graduate School of Science and Engineering, Saitama University, 255 Shimo-okubo, Sakura ku, Saitama, 338-8570, Japan. (phone/fax: (+81) 48-858-3116; e-mail: nyima.eimon@gmail.com). S. Hamamoto, is with Graduate School of Science and Engineering, and Institute for Environmental Science and Technology, Saitama University, 255 Shimo-okubo, Sakura ku, Saitama, 338-8570, Japan. (e-mail: hamasyo@mail.saitama-u.ac.jp) K. Kawamoto is with Graduate School of Science and Engineering, and Institute for Environmental Science and Technology, Saitama University, 255 Shimo-okubo, Sakura ku, Saitama, 338-8570, Japan. (e-mail: kawamoto@mail.saitama-u.ac.jp). T. Komatsu is with Graduate School of Science and Engineering, and Institute for Environmental Science and Technology, Saitama University, 255 Shimo-okubo, Sakura ku, Saitama, 338-8570, Japan. (e-mail: komatsu@mail.saitama-u.ac.jp). P. Modrup is with Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, 9000 Aalborg, Denmark. (e-mail: pm@bio.aau.dk). permeability of kaolin clay were not significant within the studied temperature range between 5 °C and 40 °C.

Efficient access to highly enantioselective isotope-labeled serine, cysteine, and alanine for stereoarray isotope labeling (SAIL) is described.