Abstract
Internal translational initiation of the mRNA encoding the Arf tumor suppressor yields an N-terminally truncated small Arf protein (smArf) that lacks amino acid residues required for Mdm2 binding and p53 activation. Here, we report that female, but not male, mice engineered to produce only smArf in lieu of the full-length Arf protein retain residual, sexually dimorphic tumor suppressive activity.
Highlights
Like Interleukin-7-dependent Arf-null or Trp53null pro-B cells, pro-B cells cultured from small Arf protein (smArf) mice are capable of continuous cytokine-dependent self-renewal
To determine whether the sex of recipient animals might play any role in determining the rate of onset and clinicopathological signs of disease, Bcr-Abl+ pro-B cells from either Arf-null or smArf male or female donor mice were infused into healthy syngeneic WT recipients of either sex (Figure 1B)
Because of the highly aggressive nature of this acute lymphoblastic leukemia (ALL) model [4, 5], we infused limiting numbers of green fluorescent protein (GFP)marked leukemia-initiating cells in an attempt to magnify any differences between eight individual cohorts of 12 mice each (Figure 1B)
Summary
Like Interleukin-7-dependent Arf-null or Trp53null pro-B cells, pro-B cells cultured from smArf mice are capable of continuous cytokine-dependent self-renewal. Males of both strains and Arf-null females developed tumors with mean latencies of 16-17 weeks, as previously reported for Arfnull mice of either sex [3].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
