Abstract
Clonorchiasis caused by Clonorchis sinensis is endemic in East Asia; approximately 15 million people have been infected thus far. To diagnose the infection, serodiagnostic tests with excellent functionality should be performed. First, 607 expressed sequence tags encoding polypeptides with a secretory signal were expressed into recombinant proteins using an in vitro translation system. By protein array-based screening using C. sinensis-infected sera, 18 antigen candidate proteins were selected and assayed for cross-reactivity against Opisthorchis viverrini-infected sera. Of the six antigenic proteins selected, four were synthesized on large scale in vitro and evaluated for antigenicity against the flukes-infected human sera using ELISA. CsAg17 antigen showed the highest sensitivity (77.1%) and specificity (71.2%). The sensitivity and specificity of the bacterially produced CsAg17-28GST fusion antigen was similar to those of CsAg17 antigen. CsAg17 antigen can be used to develop point-of-care serodiagnostic tests for clonorchiasis.
Highlights
Clonorchiasis is an infectious disease caused by a liver fluke, Clonorchis sinensis, and is endemic in East Asian countries, including China, Korea, Russia, and Vietnam
Human clonorchiasis is a parasitic disease caused by the Chinese liver fluke, Clonorchis sinensis
Humans are infected through eating raw freshwater fishes carrying C. sinensis
Summary
Clonorchiasis is an infectious disease caused by a liver fluke, Clonorchis sinensis, and is endemic in East Asian countries, including China, Korea, Russia, and Vietnam. More than 200 million people are at risk of C. sinensis infection worldwide, and 15 million people have been infected in these countries far [1,2]. Ingestion of raw or inadequately cooked freshwater fish carrying C. sinensis metacercariae causes clonorchiasis. C. sinensis infections induce pathologic changes in the biliary tree, resulting in inflammation, hyperplasia of the biliary epithelium, periductal fibrosis, cholangitis, and cholangiectasis. C. sinensis along with Opisthorchis viverrini has been classified as a group I biological carcinogen causing cholangiocarcinoma [7,8]
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