Abstract
Excretory/secretory (E/S) antigen of adult Fasciola gigantica was used for diagnosis of fasciolosis in cattle and buffaloes sera from slaughter houses and different places in and around Chennai, Tamil Nadu. A total of 6.4mg of E/S antigen was obtained from the 60 flukes collected from slaughter houses. Coprological examination was done to detect endoparasitic infection in 321 fecal samples. Dot-enzyme immuno assay (Dot-EIA) was standardized with optimum working dilution of E/S antigen, serum, and conjugate by the checker board titration as 1 in 10 (50ng/ml), 1 in 20, and 1 in 1,000, respectively. A total of 321 sera samples consisting of 168 cattle and 153 buffaloes were tested by Dot-EIA. A total of 25 cattle (14.88%) and 38 buffaloes (24.83%) showed positive results in Dot-EIA. Sensitivity of Dot-EIA for cattle and buffaloes sera was 100%. Specificity of Dot-EIA for cattle and buffaloes was 95.28 and 92.10%, respectively in comparison to fecal examination by sedimentation method. Dot-EIA can be easily adapted in the field to detect fasciolosis in cattle and buffaloes.
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