Abstract
Three serine protease inhibitors (AEBSF, soy inhibitor, α1-antitrypsin) were covalently immobilized on the surface of three polymer prostheses with the optimized method. The immobilization efficiency ranged from 11 to 51%, depending on the chosen inhibitor and biomaterial. The highest activity for all inhibitors was observed in the case of immobilization on the surface of the polyester Uni-Graft prosthesis, and the preparations obtained showed high stability in the environment with different pH and temperature values. Modification of the Uni-Graft prosthesis surface with the synthetic AEBSF inhibitor and human α1-antitrypsin inhibited the adhesion and multiplication of Staphylococcus aureus subs. aureus ATCC® 25923TM and Candida albicans from the collection of the Department of Genetics and Microbiology, UMCS. Optical profilometry analysis indicated that, after the immobilization process on the surface of AEBSF-modified Uni-Graft prostheses, there were more structures with a high number of protrusions, while the introduction of modifications with a protein inhibitor led to the smoothing of their surface.
Highlights
Polymeric substances, which are used very often as biomaterials, are much more similar to human tissues than inorganic substances
At each stage of optimization process, we examined the efficiency of immobilization and inhibition activity after the immobilization process, which was crucial for choosing optimal parameters
In the case of the synthetic inhibitor AEBSF, the selection of the optimal cross-linker compound was omitted since the only functional group that could participate in the formation of the covalent bond with the carrier is the -NH2 group
Summary
Polymeric substances, which are used very often as biomaterials, are much more similar to human tissues than inorganic substances. They can be used in targeted therapies in which specific interactions between a biomaterial and patient’s cells are planned [1]. Despite the development of more advanced biomaterials, prosthetic infections (in particular, vascular prostheses) are still a serious problem for implantology. The microorganisms that are most often responsible for causing prosthetic infections include among the others coagulase-negative staphylococci (30–43%), Staphylococcus aureus (12–23%), or Gram-negative rods (3–6%).
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