Abstract

ABSTRACTReston virus (RESTV) was discovered in 1989–1990 during three connected epizootics of highly lethal viral hemorrhagic fever among captive macaques in primate housing facilities in the United States and Philippines. Currently, only one RESTV isolate from that outbreak (named Pennsylvania) has been sequenced. Here, we report the sequence of a second isolate, Reston virus/M.fascicularis-tc/USA/1990/Philippines89-AZ1435.

Highlights

  • Reston virus (RESTV) was discovered in 1989 –1990 during three connected epizootics of highly lethal viral hemorrhagic fever among captive macaques in primate housing facilities in the United States and Philippines

  • We sequenced RESTV AZ-1435, an isolate obtained from the spleen of a crab-eating macaque with signs of respiratory infection sampled during the 1990 Reston, Virginia, USA, episode of the 1989 –1990 outbreak [17, 18]

  • RESTV AZ-1435 was isolated by inoculating MA-104 cells with 10% spleen homogenate, followed by passage of MA-104 cell supernatant onto Vero E6 cells [17]

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Summary

Introduction

Reston virus (RESTV) was discovered in 1989 –1990 during three connected epizootics of highly lethal viral hemorrhagic fever among captive macaques in primate housing facilities in the United States and Philippines. RESTV isolate sequence data are sparse [14], and only a single complete [15] genome We sequenced RESTV AZ-1435, an isolate obtained from the spleen of a crab-eating macaque with signs of respiratory infection sampled during the 1990 Reston, Virginia, USA, episode of the 1989 –1990 outbreak [17, 18]. RESTV AZ-1435 was isolated by inoculating MA-104 cells with 10% spleen homogenate, followed by passage of MA-104 cell supernatant onto Vero E6 cells [17].

Results
Conclusion

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