Separation of Selected Bile Acids by TLC. VI. Separation on Cyano‐ and Diol‐Modified Silica Layers

Abstract

Seven selected bile acids: cholic acid (C), glycocholic acid (GC), glycolithocholic acid (GLC), deoxycholic acid (DC), chenodeoxycholic acid (CDC), glycodeoxycholic acid (GDC), and lithocholic acid (LC) were separated on silica gel modified by cyano and diol groups, with the use of a mobile phase: n‐hexane–ethyl acetate–acetic acid in different volume compositions, at 18°C. The estimation of separation was carried out on the basis of the separation factors values: ΔRF and RS. Almost all bile acids, except for CDC/DC, were completely separated on cyano‐modified silica plates when a mobile phase n‐hexane–ethyl acetate–acetic acid in the volume composition 49:49:2 was used; whereas, the optimal separation for all examined bile acids was obtained on diol plates, using a mobile phase n‐hexane–ethyl acetate–acetic acid in the volume composition 42:42:16.