Abstract

Capillary electrophoresis (CE) has been introduced for analysis of charged molecules. It has been introduced as high performance capillary electrophoresis (HPCE), and is a powerful method for separation of nucleic acids, proteins and peptides. In this chapter, we also describe the application of HPCE for separation of PTH amino acids after protein sequencing or protein hydrolysis. The wide range of different separation conditions, including: buffers pH, temperature, ion-forming reagents, micelle-forming reagents, complex reagents, packed capillaries, makes the separation of different bio-molecules possible.

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