Abstract
A method was developed for the separation of nitrite from food products by ion-exchange column chromatography. The sample was extracted with water at pH 9.0, and the filtrate was transfered to a column of Amberlite IRA-410 (Cl form). Nitrite was eluted with solvent system consisting of methanol and 2% sodium chloride solution. Nitrite in the effluent was determined by colorimetry using sulfanilamide and N-naphthylethylenediamine. Recovery of added nitrite from various food products was 87-98%. Ion-exchange chromatography was found to be an efficient and reproducible technique for removing extraneous materials from extracts.
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