Abstract
Two Chromatographic methods have been developed to separate quantitatively a number of organic compounds such as alloxan, urea, allantoin, parabanic acid, oxaluric acid and D-ribose from relatively large amounts of inorganic phosphate. These organic compounds are representative of typical products expected upon electrochemical oxidation of various purine derivatives which may be themselves separated from each other by liquid chromatography with phosphate buffers. The phosphate may subsequently be separated from the organic components either by use of a Sephadex G-10 gel permeation column with water or very dilute hydrochloric acid as the eluant or by use of a methanol-washed column of a strong cation-exchange resin with methanol as the eluant.
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