Abstract
THE separation of phospholipids by paper chromatography has generally been performed on modified papers. Marinetti and Stotz1 and Lea et al.2 report the use of silicic acid-impregnated paper, and Brown et al.3 have used silicic acid-impregnated glass-fibre ‘paper’. Rouser et al.4 investigated the possibility of using paper impregnated with mineral oil, rubber, silicone, and formamide, and paper oxidized by periodic acid, but found all these to be unsuitable. Recently, however, in this laboratory5 a method has been evolved for the separation and differential staining of phospholipids on untreated paper. During work on this method difficulty was encountered with the separation of lecithin from phosphatidyl ethanol-amine, and a procedure for separating them on paper impregnated with zinc salts is described here.
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