Abstract
The retention behavior of inorganic anions on a pyridine stationary phase commercially available for hydrophilic interaction chromatography was examined in ion chromatography. Inorganic anions were retained on a protonated pyridine stationary phase under acidic eluent conditions (pH 3.1-3.3) in the ion-exchange mode. The logarithm of the retention factor of analytes was linear to the logarithm of the eluent concentration, and the slopes of the plots were -0.55 to -0.64, except for nitrite (-0.39). The smaller slope for nitrite was due to the fact that nitrous acid is weak (pK(a) 3.25 at 25 degrees C) and was partially ionized under the operating conditions. The elution order of the examined anions was the same as that observed in common ion chromatography. The present system was applied to the determination of UV-absorbing anions contained in saliva.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
