Abstract
1. 1. Galactose 1-phosphate is easily separated from the other common hexose phosphates in the chromatographic system of Goodman, Benson and Calvin (Dowex-1 borate column, borax eluant). It emerges from the column after glucose 1-phosphate and before fructose 6-phosphate. Mannose 6-phosphate is eluted with fructose 6-phosphate and galactose 6-phosphate with glucose 6-phosphate. 2. 2. Procedures are described for the removal of borate from chromatographic fractions containing hexose phosphates, and for the subsequent isolation of the phosphates as salts. 3. 3. A sensitive, convenient colorimetric method for monitoring chromatograms of sugars and their derivatives is described. The method discriminates well between hexoses and pentoses. 4. 4. Instructions are given for the accurate determination of inorganic phosphate in the presence of hexose 1-phosphates.
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