Abstract
α- and β-cyclodextrins have been immobilized on polyacrylamide gel beads via two spacers. The resulting stationary phases containing α-cyclodextrin exhibit selectivity in the liquid chromatographic separation of disubstituted benzene isomers as models: the retention order is o <m <p in all cases. Except for aminobenzoic acid, the o-, m- and p-isomers of each solute tested can be completely separated on the α-cyclodextrin stationary phases. The β-cyclodextrin stationary phases also interact more strongly with the p- than with the o- or m-isomers. In the case of dinitrobenzene, the o-isomer is retained most strongly.
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