Abstract
A fast and efficient procedure for preparation of indigotin and indirubin from Folium isatidis extracts using macroporous resin followed by reversed phase flash chromatography was investigated in this paper. Nine widely used macroporous resins were used to enrich and separate two alkaloids from Folium isatidis. Finally, D3520 resin was chosen to enrich two alkaloids through static and dynamic adsorption tests. After one run of adsorption and desorption, the contents of indigotin and indirubin were 15.2 and 15.5-fold increased with recovery yields of 87.8% and 89.7%, respectively. The enriched sample was directly subjected to flash chromatography on C18 reversed phase silica gel column, and the separation of indigotin and indirubin was dramatically improved by using ionic liquid as mobile phase modifier for decreasing band tailing, reducing band broadening, and improving resolution. Indigotin and indirubin with purities of more than 96% were produced with recovery yields of 90.1% and 92.0% by C18 reversed phase flash chromatography. The developed procedure boasts production of high-purity product as well as high recoveries, and allows an easy scale-up. This study provides an excellent basis for large-scale preparation of alkaloids from Folium isatidis or other plant extracts.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of the Taiwan Institute of Chemical Engineers
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
