Abstract
AbstractThe on‐line high‐performance liquid chromatography‐electrochemistry‐thermospray mass spectrometry (LCEC‐LC/MS) for separation and identification of the phenolic isoflavones genistein, formononetin, and Biochanin A is described. The isoflavones are separated on a C‐18 reversed phase column with methanol: 0.1 M ammonium acetate pH (70:30 v/v) as eluent, which is compatible for both LCEC and LC/MS detection. Low ng detection is achieved with a conventional glassy carbon thin‐layer electrochemical cell at 1.3 V vs. Ag/AgCl. Selected ion monitoring (SIM) mode LCMS results in detection levels at the 20–50 ng range, and the compounds are positively identified. In the on‐line detection, the electrochemical cell is connected to the thermospray and the column effluent flows from the working electrode into the heated thermospray capillary tube. The results show that on‐line coupling between a thin‐layer LCEC and a thermospray‐LC/MS can be used to characterize analytical systems.
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