Abstract
Milling of wood samples is a widely applied preparation method for pooling tree-rings from different trees or periods of several years for determination of δ(13)C and δ(18)O values. In this study, whole wood samples were milled using different procedures in order to evaluate potential effects of this preparation method on δ(13)C and δ(18)O values. Subsamples of a 5 cm(3) wood piece of a single tree-ring from a lowland white fir were used. The samples were milled with different setups: (i) two and three stainless-steel balls, (ii) 3, 5 and 8 min milling time, and (iii) discontinuous and continuous milling. The δ(13)C values were measured using an elemental analyser connected to an IsoPrime mass spectrometer and δ(18)O values using a Thermo Scientific MAT 253 mass spectrometer and a TC/EA connected by a ConFlo IV. The results show that varying the milling procedure does not alter the δ(13)C and δ(18)O values in comparison to non-milled blank samples. For shorter milling times, an increased variance of δ(18)O values is recorded, probably caused by isotopic gradient between early- and latewood portions of the tree-ring and thereby biasing the insufficiently homogenised samples. No overheating effects on the δ(13)C and δ(18)O values were detected. Milling of wood samples for carbon and oxygen isotope analyses is an appropriate preparation method.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.