Sensitivity analysis of immunohistochemical examination for ROS1 rearrangement compared to RT-PCR in patients with lung adenocarcinoma

Apathy in Dementia: An Examination of the Psychometric Properties of the Apathy Evaluation Scale

Sambit K. MohantyObjective Repressor of Silencing (ROS1) gene rearrangement in the lung adenocarcinomas is one of the targetable mutually exclusive genomic alteration. Fluorescence in situ hybridization (FISH), immunohistochemistry (IHC), next-generation sequencing, and reverse transcriptase polymerase chain reaction assays are generally used to detectROS1gene alterations. We evaluated the correlation betweenROS1IHC and FISH analysis considering FISH as the gold standard method to determine the utility of IHC as a screening method for lung adenocarcinoma.Materials and Methods A total of 374 advanced pulmonary adenocarcinoma patients were analyzed forROS1IHC on Ventana Benchmark XT platform using D4D6 rabbit monoclonal antibody. FISH assay was performed in parallel in all these cases using the VysisROS1Break Apart FISH probe.Statistical Analysis The sensitivity, specificity, positive and negative likelihood ratios, positive and negative predictive values, and accuracy were evaluated.Results A total of 17 tumors were positive either by IHC or FISH analysis or both (true positive). Four tumors were positive by IHC (H-score range: 120–270), while negative on FISH analysis (false positive by IHC). One tumor was IHC negative, but positive by FISH analysis (false negative). The sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, positive predictive value, negative predictive value, and accuracy were 94.4% (confidence interval [CI]: 72.71–99.86%), 63.6% (CI: 30.79–89.07%), 2.6 (CI: 1.18–5.72), 0.09 (CI: 0.01–0.62), 80.95% (CI: 65.86–90.35%), 87.5% (CI: 49.74–98.02%), and 82.76%, respectively.Conclusion ROS1IHC has high sensitivity at a cost of lower specificity for the detection ofROS1gene rearrangement. All IHC positive cases should undergo a confirmatory FISH test as this testing algorithm stands as a reliable and economic tool to screenROS1rearrangement in lung adenocarcinomas.

Diagnostic indexes of a rapid immunoglobulin G/immunoglobulin M combined antibody test for severe acute respiratory syndrome coronavirus 2.

The frequency and impact of ROS1 rearrangement on clinical outcomes in never smokers with lung adenocarcinoma

A Single-Tube Multiplexed Assay for Detecting ALK, ROS1, and RET Fusions in Lung Cancer

Predicting Post-Heart Transplant Composite Renal Outcome Risk in Adults: A Machine Learning Decision Tool

SARS-CoV-2 IGM and IGG rapid serologic test for the diagnosis of COVID-19 in the emergency department

Influence of Age on the Outcome of the Atopy Patch Test with Food in Children with Atopic Dermatitis

P1.03-082 18F-FDG PET/CT Value Staging NSCLC Extension to the Lymph Nodes, Single Center Experience

Objective To investigate the expression level of lipoprotein lipase (LPL) mRNA in chronic lymphocytic leukemia (CLL) patients and evaluate the prognostic value of LPL in CLL Methods Quantitative real-time RT-PCR (qRT-PCR) was performed in 62 CLL patients, 10 normal controls using Taqman probe system. Association between LPL and other known prognostic factors, such as IgVH mutation status, ZAP-70 and CD38 expression, was determined using the Spearman correlation analysis. ROC curve was used to determine the cut-off value of LPL expression level, the positive and negative predictive value of IgVH mutation status. Results The correlation coefficients of the standard curves in qRT-PCR were not less than 0.990. The coefficients of variation (CV) of interrun assay and intramn assay were < 5%, and the sensitivity can reached 102 copies/μg RNA. The median LPL mRNA expression level was 0.006 0 (0-0.737 0) in 62 CLL patients, whereas in 10 normal controls LPL mRNA expression level was extremely low with the median level of 0 (0-0.000 4). The expression levels of LPL in three CLL samples after miniMACS-sorted CD19 positive B cells were 0.036 0, 0.075 0 and 0.197 0, which were similar to the levels before miniMACS-sorted (0.024 0, 0.074 0 and 0.225 0). LFL expression was significantly associated with IgVH mutation status (r=0.45, P<0.05) . LPL expression level in IgVH unmutated patients [0.006 0 (0.000 7-0.110 0)] was significantly higher than the level in IgVH mutated patients [0.002 0(0.000 2-0.027 0)] (U=96.5, P<0.05). LPL expression was also significantly associated with ZAP-70 (r=0.38, P<0.05), CD38 expressions (r=0.43, P<0.05). According to ROC curve, the cut-off of LPL mRNA expression level was 0.036, with a 66.7% specificity, a 72.4% sensitivity, a 51.8% positive predictive value (IgVH unmutated), and a 83.3% negative predictive value (IgVH mutated) for IgVH mutation status. Conclusions The qRT-PCR assay is reliable and sensitive. LPL mRNA expression significantly correlates with IgVH mutation status, ZAP-70 and CD38 expression, and could be a predictive marker of IgVH mutation status. Our data confirms a role for LPL as a novel prognostic indicator in CLL. Key words: Leukemia, lymphocytic, chronic, B-cell; Lipoprotein lipase; Reverse transcriptase polymerase chain reaction; Prognosis

Objective To explore the clinical application value of fluorescent immunoassay kit for influenza virus antigen detection by comparing the method to the direct fluorescent antibody staining and the reverse transcriptase polymerase chain reaction (RT-PCR) of influenza virus nucleic acids. Methods From July to December 2017, 350 patients in the fever clinic were examined by the fluorescent immunoassay kit for influenza virus antigen detection (Sofia FIA), direct fluorescent antibody staining (DFA) and RT-PCR of influenza virus nucleic acids. Results A total of 186 cases of influenza A/B viruses were detected by Sofia FIA, with an overall positive detection rate of 53.1%, and the detection rates of influenza A and B viruses were 35.4% and 17.7%, respectively. Compared to RT-PCR, the results of sensitivity, specificity, positive predictive value, negative predictive value, consistency rate and kappa value of Sofia FIA were 89.4%, 100.0%, 100.0%, 86.6%, 93.7% and 0.873 in the combined detection of influenza A/B viruses; 87.9%, 100.0%, 100.0%, 92.5%, 95.1% and 0.897 in the detection of influenza A virus; and were 92.5%, 100.0%, 100.0%, 98.3%, 98.6% and 0.952 in the detection of influenza B virus, respectively. Compared to RT-PCR, the results of sensitivity, specificity, positive predictive value, negative predictive value, consistency rate and kappa value of DFA were 83.2%, 100.0%,100.0%, 80.2%, 90.0% and 0.800 in the combined detection of influenza A/B viruses; 82.3%, 100.0%, 100.0%, 89.3%, 92.9% and 0.847 in the detection of influenza A virus; and were 85.1%, 100.0%, 100.0%, 96.6%, 97.1% and 0.902 in the detection of influenza B virus, respectively. Conclusions Sofia FIA and DFA had high sensitivity and specificity, and were in consistency with RT-PCR. The methods showed have certain clinical significance in the screening of influenza. Key words: Influenza; Rapid detection of influenza virus; Fluorescent immunoassay; Diagnosis

For clinicians involved in investigating and treating sleep disorders, understanding the accuracy of patient recall of supine sleep would allow informed comparisons between polysomnography (PSG) and patient-reported sleep in patients with supine-predominant obstructive sleep apnea. This study aims to assess the accuracy of patient perception of supine sleep. Prospective observational cohort study, assessing patient perception of total sleep and supine sleep, including duration. Data were analyzed utilizing descriptive statistics, bias-plot (Bland-Altman) analysis, and Spearman correlation (rs) to analyze relationships among continuous data. Total number of patients who underwent PSG was 518, with data from 368 of these patients analyzed. Most of these patients underwent diagnostic PSG (49.2%). Patients were excluded because of missing or incomplete data (n = 133) or immobility (n = 17). Some patients (n = 97, 26%) did not perceive supine sleep, with 34 (35% of those with unperceived supine sleep or 9% of whole group) of these having more than 60 minutes of PSG supine sleep (range 0-305.5 minutes). All "unsure" patients (n = 8, 2.2%) had significant supine sleep recorded (31.5-257.5 minutes). For the presence of any PSG supine sleep, questioning had a sensitivity of 77.9%, specificity 72.7% with positive predictive value of 96.7% and negative predictive value of 24.5%. There was a significant correlation (rs = 0.63, P < .0001) between perceived and PSG supine sleep, but wide limits of agreement (-246.9 to 194.2 minutes). In patients undergoing in-laboratory PSG, the perception of supine sleep predicts the presence of PSG supine sleep. However, questioning patients has a poor negative predictive value and patient estimates of supine sleep duration are inaccurate.

Objective To investigate the specific chest computed tomography (CT) features of corona virus disease 2019(COVID-19) and to evaluate its clinical diagnostic value. Methods The clinical data of 35 cases with suspected novel coronavirus pneumonia diagnosed from Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine and Oriental Hospital affiliated to Shanghai Tongji University School of Medicine from January 1, 2020 to February 14, 2020 were retrospectively analyzed. A total of 17 cases with positive results of two times of real time reverse-transcription–polymerase chain-reaction (RT-PCR) for 2019 novel coronavirus (2019-nCoV) were evaluated as the case group, and the remaining 18 cases with negative results of two times of RT-PCR for 2019-nCoV were evaluated as the control group. The features of chest CT images of 35 cases were obtained. The frequencies of four CT imaging indicators including ground-glass opacities (GGO), crazy-paving , heterogeneous consolidation and bilateral subpleural distribution were analyzed. The sensitivity, specificity, positive predictive values (PPV) and negative predictive value (NPV) for COVID-19 were calculated. Results In the case group, there were 11 cases (64.71%) with GGO, 7 cases (41.18%) with crazy-paving, 6 cases (35.29%) with heterogeneous consolidation, and 16 cases (94.12%) with bilateral subpleural distribution, while in the control group, there were 7 cases (27.28%) with GGO, 1 case (5.56%) with crazy-paving, 6 cases (33.33%) with heterogeneous consolidation, and 5 cases (29.41%) with bilateral subpleural distribution. When multiple subpleural lesions or any two of the CT indicators were used as the characteristic indicator, the diagnosis efficiencies were better, with the sensitivity, specificity, PPV, NPV and Youden index of 94.12%, 72.22%, 76.19%, 98.86% and 0.66, respectively, and 88.24%, 77.78%, 78.95%, 87.5% and 0.66, respectively. Conclusions Chest CT indictors are of high clinical diagnostic value for COVID-19. Any two of the four CT indicators (GGO, crazy-paving, heterogeneous consolidation and bilateral subpleural distribution) or the single characteristics (bilateral subpleural distribution) are of high diagnostic efficacy. Key words: COVID-19; Computed tomography

Psychosocial and psychiatric aspects of marginal youth group study

Dépistage des troubles de la personnalité avec la version française du Personality Diagnostic Questionnaire-4+ dans une population psychiatrique : une étude préliminaire