Abstract
A rapid LC method with UV detection was developed for the quantification of carboxylic acid metabolite of clopidogrel in human plasma. Following a simple protein precipitation using a mixture of methanolic solution of ZnSO4, the analyte and commercially available internal standard were separated using a mobile phase of water–acetonitril (85:15, v/v) adjusted to pH 3.5 on a Chromolith C18 column at a flow rate of 2.5 mL min−1 with a total retention time of 4 min. Linearity was verified over the range of 20–3,000 ng mL−1 where the LOQ was 20 ng mL−1. This method was applied in a pharmacokinetic study.
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