Abstract
A selective and sensitive quantitative method is described for the determination of the endogenous prostacyclin content of the aorta of rats, treated with GYKI-11679, a new hydrazone derivative with antihypertensive action. The thoracal aorta from three rats was excised and pooled, 1 h after treatment with 10 mg/kg of GYKI-11679. The prostacyclin, transformed to 6-oxo-PGF 1α, was extracted. For gas chromatographic electron-capture detection (GC-ECD), 6-oxo-PGF 1α, was converted into the O-methyloxime tris(trifluoroacetyl) hexafluoroisopropyl ester derivative. The GC-ECD properties, stability and mass-spectrometric characteristics of this new derivative allow the quantitative analysis of 6-oxo-PGF 1α, by using nor-PGF 2α as internal standard.
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