Abstract
In this work, blue-emitting chicken cartilage carbon dots (cc-CDs) were synthesized using biomass as green precursors. The abundant heteroatoms in biomass allowed for self-doping during the synthesis of cc-CDs. The experimental results showed that cc-CDs possessed excellent optical properties and good stability. The elemental composition, graphitized structure and surface groups of cc-CDs have characterized by various instruments. In addition, the low cytotoxicity of cc-CDs was demonstrated by the high viability of cells which incubated with cc-CDs solutions. Subsequently, a sensitive and accurate biosensor for the determination of H2O2 was established using cc-CDs as fluorescent probe. In a departure from previous reports, fluorescence quenching was confirmed to be caused by the dual effects of Fe3+ and hydroxyl radicals rather than the single effect of Fe3+. The method for detection of H2O2 exhibited good selectivity, wide linear range (2–500 μM, 500–1500 μM) and low detection limit (0.47 μM). In addition, the biosensor could be used to detect glucose in the presence of glucose oxidase (GOx). The sensing system was further applied to the detection of glucose in human serum, and satisfactory results were obtained.
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