Abstract
A sensitive lime resolved registration of agglutinates of resuspended platelets under the action of definite flow conditions is achieved by means of a cylinder-cylinder shear cuvette in combination with the measurement of ultrasound scattering. ADP induced platelet agglutination is reliably measurable with ultrasonic scattering. Platelet agglutinates in PRP (platelet rich plasma) are detectable by ultrasonic scattering already around mean shear rates of 400 s−1. Comparing the methods of light transmission and ultrasound scattering for the detection of weak agglutinations shows that ultrasonic scattering is more sensitive. In contrast to PRP, washed platelets scatter ultrasound weakly but detectably at low shear rates. Because of enhanced sensitivity, the developed measurement system is capable to investigate shear induced receptor mediated GpIb-vWF agglutinations. In addition, agglutinations mediated by GpIIb-IIIa-fibrinogen can be used for a test of the action of anticoagulantis at low shear rates in order to exclude the effect of self activation of platelets by shear forces.
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