Abstract
A novel amine-functionalized silica quantum dots (SiQDs) fluorescent nanoprobe was developed for sensing of lead concentration in water, plasma and cell lysate. In addition, the developed probe was utilized for bioimaging of intracellular lead ions in HT 29 cancer cells. The amine-functionalized nanoprobe exhibited fluorescence emission at 445 nm under excitation at 355 nm. Upon addition of lead ions, the fluorescence of SiQDs linearly enhanced from 50 ng/mL to 5 µg/mL and 50 ng/mL to 25 µg/mL for plasma and standard media, respectively. The synthesis and fabrication of this probe are simple and serves high sensitivity with a limit of detection down to around 20 ng/mL. In the presence of various molecular and ion interfering, reliable results are obtained, confirming the specificity of the nanoprobe for lead ion detection. Meanwhile, amine-functionalized SiQD-based nanoprobe exhibits excellent cell membrane-permeability and biocompatibility. Thus, this probe is utilized for lead tracing in HT 29 cancer live cells. Fluorescent microscopy results confirmed the attachment of the produced nanomaterials to the HT 29 cancer cells.
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