SEMINAR FORMULASI SEDIAAN PASTA GIGI EKSTRAK DAUN PANDAN WANGI (Pandanus amaryllifolius Roxb) DAN UJI EFEKTIVITAS TERHADAP BAKTERI Streptococcus mutans

Pandan Wangi leaves (Pandanus amaryllifolius Roxb) contain flavonoid chemicals found in fragrant pandan leaves which have antimicrobial, anticancer, antidiabetic, and lower cholesterol levels. The purpose of this study was to determine the effect of fragrant pandan leaf extract (Pandanus amaryllifolius Roxb) in lowering cholesterol in mice (Mus musculus) and to determine what dose of fragrant pandan leaf extract could lower cholesterol well in mice. The research method used is an experimental method using a maceration extraction process. This study used 15 mice (Mus musculus), then given fragrant pandan leaf extract (Pandanus amaryllifolius Roxb) with dosage variations of 40 mg/kgBW, 80 mg/kgBW, 160 mg/kgBW, negative control (Na.CMC), and a positive control (Simvastatin) previously induced by Alloxan. Cholesterol level examination was carried out twice, namely after Alloxan was induced and after being treated using a Nesco GCU measuring instrument. The result of this research is that pandan fragrant leaf extract (Pandanus amaryllifolius Roxb) can reduce cholesterol levels in the blood of mice (Mus musculus) where a dose concentration of 80 mg/kgBW shows the best dose in lowering cholesterol levels.

The fragrant pandan leaf (Pandanus amaryllifolius Roxb.) contains flavonoid compounds that can function as a sunscreen. Sunscreen preparations are generally in the form of creams. In cream formulations, emulsifiers play a crucial role in imparting physical properties to the cream. The objective of this research is to determine the influence of the combination of stearic acid and triethanolamine on the physical properties and stability of the cream and to identify the optimal emulsifier concentration. Cream formulations were prepared in three formulations with a combination of stearic acid and triethanolamine (TEA), namely F I (17%: 3.5%), F II (18%: 3%), and F III (19%: 2.5%). The cream formulations underwent physical evaluations such as organoleptic testing, homogeneity testing, viscosity testing, adhesion testing, pH testing, skin irritation testing, spreading power testing, and stability testing. The data obtained were analyzed using One Way ANOVA (analysis of variance) with a confidence level of 95%. The research data results showed that the addition of stearic acid and trietanolamin affected the viscosity, pH value, spreading power, and adhesion of the cream but did not affect the organoleptic properties, stability, homogeneity, and skin irritation. Based on the physical properties and statistical tests, the concentration of stearic acid and trietanolamin emulsifier (17%: 3.5%) was found to be the best.

Sleep plays a role in restoring the body's biochemical and physiological processes which decrease when awake. People is not familiar yet with sleep disturbances, so they rarely seek help. Sleep disturbances can cause family problems and are at risk of fatal traffic accidents. Fragant Pandan leaves (Pandanus amaryllifolius Roxb.) contains alkaloids, saponins and flavonoids which can provide a hypnotic sedative effect. The purpose of this study was to determine the effect of fragant Pandan leaf extract (Pandanus amaryllifolius Roxb.) administration in shortening the sleep induction time of male Swiss Webster strain mice. A post-test only design experimental laboratory study was conducted with control group design. The test animals used were 25 Swiss Webster male mice divided into five groups randomly : G1 group was given distilled water, G2 was given diazepam dose 1 mg / KgBB, the G3, G4 and G5 groups were given fragant Pandan leaf extract with a dose of 4 mg/25g BB, 6 mg/25g BB and 8mg/25g BB, respectively. One Way ANOVA statistical test showed significant differences in mice sleep onset (p=0.000) between groups. The results of the Post Hoc test using LSD analysis showed that there were significant differences between positive controls with groups G3, G4 and G5. Fragrant Pandan leaf extract dose of 8 mg/g BB can shorten the time of mice sleep induction male Swiss Webster strain mice. While the dose of 4 mg/g BB cannot shorten the time of induction of sleeping mice.
 Keywords: Fragant Pandan leaf extract; Hypnotic-sedative; Sleep disturbances.

ABSTRACTDysentriae Shigella is a bacterial species of shigella that occurs in tropical countries, these bacteria are pathogenic gut bacteria are commonly known to cause dysentery. Dysentery is an infection that causes sores that cause ulcers limited dicolon characterized by symptoms known as dysentery syndrome. In user of trimatoprim sulfametaksazol can cause or accelerate the onset of megaloblastosis, leocopenia, or trombostopenia. In regular use, this combination appears to show little toxicity, approximately 75% of these adverse effects involving the skin. ‘Pandan wangi’ (pandanus amaryllifolius roxb) is one of the plants that can be used as a natural antibiotic against shigella dysentriae infection because the content of fragrant pandan leaves include flavonoids, alkaloids, saponins and tannins.This type of research is experimental, while the goal is to determine the effect of ‘pandan wangi’ leaf (pandanus amaryllifolius roxb) juice on shigella dysentriae. Consisting of 7 concentrations and four repetitions. Using dilution method by observing the growth of bacteria shigella dysentriae on ‘pandan wangi’ leaf juice that is characterized by the colonies grown on media Mac Conkey (MC).Data analysis uses Anova with an error or a = 5%. The advanced test uses Tukkey test, Honestly Significant Difference (HSD). After doing the research, the optimal concentration of juice of ‘pandan wangi’ (pandanus amaryllifolius roxb) leaves can inhibit shigella dysentriae is 25% and can kill shigella dysentriae from concentration of 50%. The final conclusion is that there is the effect of ‘pandan wangi’ (pandanus amaryllifolius roxb) leaf juice on shigella dysentriae. Keywords: Pandanus amaryllifolius Roxb leaves, shigella dysentriae.

Scrub cream is a pharmaceutical preparation which function is to remove dead skin cells, moisturize and brighten the skin with the help of scrub ingredients. One of the natural ingredients used as raw material for scrub cream is fragrant pandan (Pandanus amaryllifolius Roxb.). The aim of this study was to make a cream scrub preparation from pandan extract with various concentrations of 3%, 3.5% and 4%. This research was conducted experimentally. Preparation testing includes organoleptic test, homogeneity test, pH test, irritation test, spreadability test, stability test, and emulsion type test. The results of the physical quality inspection of the scrub cream from pandan Wangi extract showed that the preparation was homogeneous, had an M/A emulsion type, had a pH value of 6.1-6.5, did not cause irritation and did not change during storage for 4 weeks except for the preparation with a concentration of 3.5% because the preparation underwent a change in the breakdown of the emulsion in the preparation on storage starting from the 3rd week. From the research that has been done, it can be concluded that Pandanus amaryllifolius Roxb extract can be formulated as a scrub cream with a variation of concentration of 3%; 3.5% and 4% and does not irritate the volunteers' skin.

The prohibition of Antibiotic Growth Promoters (AGP) has encouraged the use of natural feed additives such as pandan leaf (Pandanus amaryllifolius Roxb) water extract. This study aims to evaluate the effect of administering pandan leaf water extract in drinking water on broiler performance. A total of 100 one-day-old broiler chicks with uniform body weight were randomly divided into 4 treatment groups, each with 5 replicates, and each replicate consisted of 5 broilers. The treatments included broilers provided with drinking water without pandan leaf extract (P0), while groups P1, P2, and P3 were given drinking water containing 3%, 6%, and 9% pandan leaf water extract, respectively. The results showed that the addition of pandan leaf water extract at levels of 3%, 6%, and 9% had no significant effect (P>0.05) on feed intake, water intake, final body weight, weight gain, and Feed Conversion Ratio (FCR). It was concluded that the addition of pandan leaf water extract up to a level of 9% in drinking water does not influence broiler performance, however there is a trend of increasing feed efficiency when adding 3-6% pandan leaf water extract to broiler drinking water.

Abstract: Flies are vectors of foodborne diseases, among others, vomiting, diarrhea, typhoid, dysentery, and myiasis. Therefore, flies need to be controlled, one of the methods to control flies is using natural pesticides. The purpose of this study was to determine the differences in the effectiveness of decoction of clove leaves (Syzygium aromaticum L) and pandan leaves (Pandanus amaryllifolius Roxb) as a vegetable repellent for house flies (Musca domestica). This type of research was true experiment with a Posttest Only Control Group Design design. The samples in this research were the house fly (Musca domestica) with the number 315 tail and repetition 3 times. The results showed that there was a difference in effectiveness between the decoction of clove (Syzygium aromaticum L) and pandan (Pandanus amaryllifolius Roxb) leaves. With the difference power repellent repellent in the two types of repellent, namely repellent the clove leaf concentration of 40% was 91.46% and repellent pandan leaf concentration of 40% was 92.36%. From the results of this study, it is advisable for the public to use a decoction of pandan leaves (Pandanus amaryllifolius Roxb) as an repellent house fly (Musca domestica) environmentally friendly and further research can be carried out on carrier insects vector other.

Abstract: Flies are vectors of foodborne diseases, among others, vomiting, diarrhea, typhoid, dysentery, and myiasis. Therefore, flies need to be controlled, one of the methods to control flies is using natural pesticides. The purpose of this study was to determine the differences in the effectiveness of decoction of clove leaves (Syzygium aromaticum L) and pandan leaves (Pandanus amaryllifolius Roxb) as a vegetable repellent for house flies (Musca domestica). This type of research was true experiment with a Posttest Only Control Group Design design. The samples in this research were the house fly (Musca domestica) with the number 315 tail and repetition 3 times. The results showed that there was a difference in effectiveness between the decoction of clove (Syzygium aromaticum L) and pandan (Pandanus amaryllifolius Roxb) leaves. With the difference power repellent repellent in the two types of repellent, namely repellent the clove leaf concentration of 40% was 91.46% and repellent pandan leaf concentration of 40% was 92.36%. From the results of this study, it is advisable for the public to use a decoction of pandan leaves (Pandanus amaryllifolius Roxb) as an repellent house fly (Musca domestica) environmentally friendly and further research can be carried out on carrier insects vector other.

<p>Fragrant pandanus leaves (<em>Pandanus amaryllifolius</em> Roxb.) commonly used as additives in food has a compound content that includes flavonoid compounds, alkaloids, saponins, tannins, and polyphenols and dyes are suspected to contribute as antibacterial. In addition, the content contributes to other activities such as antidiabetic, chemical content in leaves that are suspected to be antibacterial are flavonoids. The purpose of this study is to find out if pandanus fragrant leaf extract (Pandanus amaryllifolius Roxb.) effectively inhibits the growth of staphylococcus epidermidis bacteria and know what concentration of fragrant pandan leaf extract (Pandanus amaryllifolius Roxb.) is effective in inhibiting the growth of staphylococcus epidermidis bacteria. The method used in this study was disc diffusion method with concentrations of 30%, 50%, 70%, 90% and 100% respectively.The results in this study pandan leaf extract fragrance (Pandanus amaryllifolius Roxb.) able to inhibit the growth of bacteria Staphylococcus epidermidis, with results obtained at a concentration of 30% obtained on average 2 mm, concentration 50% 2.3 mm, concentric 70% 9.6 mm, concentric 90% 10.6 mm and at 100% concentricity obtained the largest average of 14 mm is done each 3 repetitions. Effective concentration inhibits the growth of staphylococcus epidermidis bacteria at a concentration of 100%. Positive control used in this study is antibiotics (erythromycin) obtained an average tasteless zone of 30.3 mm in 3 repetitions while in negative control using sterile aquadest does not indicate the presence of a tasteless zone around the disc paper.</p>

Pandanus amaryllifolius Roxb. leaf has a natural mouthwash used as an alternative to commercial mouthwash. The purpose of this study was to determine the potential of giving scented Pandan leaf extract (Pandanus amaryllifolius Roxb.) using nanoemulsion technique as a natural mouthwash. This study used an experimental method with a trial of Completely Randomized Design (CRD) with five treatments and five replications. Data were analysed using Analysis of variance Test and Duncan at α=0.01 (99% confidence level). The result of giving 21-day natural mouthwash showed that P3 (4% extract) has the average salivary pH higher than the value of P1 (1% extract) and P2 (2 % extract), and the average salivary pH value of P3 (4% extract) approached the average salivary pH of P0+ (commercial mouthwash). Besides, there was a significant effect of 21-day of treatment to the respondents that is showed by F (4.20) = 20.57, ρ (0.01) = 4.43. The conclusion of this study is the administration of P amaryllifolius extract with nanoemulsion technique can be used as a natural mouthwash which is to maintain the salivary pH.

Fragrant pandanus is a type of monocot plant from the pandanaceae family. Fragrant pandan leaves have various ingredients and can be used as antibacterial ingredients and are safe to use, which have been known empirically by the Indonesian people. There are many benefits to the fragrant pandan leaf plant (Pandanus amaryllifolius Roxb), namely as an analgesic (toothache medicine) and antibacterial. The purpose of this study was to determine the chemical content, formulation and stability test in mouthwash preparations. In this study, the maceration method is used which is used to filter simplicia containing chemical components that are easily soluble in liquid. The results of this study indicate that in the phytochemical screening test there are chemical compounds which include flavonoids, alkaloids, saponins, and tannins, which are thought to have contributed to bacterial activity. Research has also been carried out on the formulation and stability test of mouthwash preparations from the ethanol extract of pandan fragrant leaves (Pandanus amaryllifolius roxb) where the results show that the formulation can be used as mouthwash of fragrant pandan leaves (Pandanus amaryllifolius roxb) with different concentrations in formulation I. as much as 15.5%, in formulation II as much as 16.5%, and in formulation III 17.5%. And the physical quality test of the mouthwash includes an organoleptic test which has a distinctive smell of fragrant pandan leaves and menthol and the pH test has a stable pH of 5. The results obtained basically produce mouthwash with good physical quality.

This study aims to formulate and evaluate the preparation of an anti-aging cream made from astaxanthin. Astaxanthin has antioxidant activity, as well as other carotenoid compounds. Astaxanthin shows more significant antioxidant activity than ?-carotene; thus,developing astaxanthin compounds in cream preparations is necessary. This type of experimental laboratory research compares three different concentrations of astaxanthin compounds, including formula I (0.5%), formula II (0.75%), and formula III (1%). Evaluation of the astaxanthin compound cream preparations stability included organoleptic observations (smell, color, texture), pH test, homogeneity test, viscosity test, spreadability test, adhesion test, and freeze-thaw test. The results of the quality inspection of organoleptic preparations revealed no difference between the preparations in the form of color, smell, and texture.The homogeneity test showed a homogeneous preparation; the pH test produced was 5.5-5.8, the viscosity test of the preparation produced was 5140 Cp, 5250 Cp, and 7000 Cp, the adhesion test was 6.40-7.83 seconds, the dispersion test was 5-5.9 cm, and the Freeze-thaw test showed that there was no significant change in the pH test, organoleptic test, spreadability, adhesion, homogeneity, and viscosity. Thus, it can be concluded that the three astaxanthin compound cream formulas have good stability.

One of the endemic plants of the island of Sulawesi, Indonesia is the Dengen plant (Dillenia serrata). One part of this plant that is claimed by the public as a treatment for vomiting blood is the bark because the stem bark contains polyphenolic compounds which are efficacious as antioxidants and sunscreens. Based on the results of the study, Dengen stem bark extract (EKBD) has been standardized and in accordance the requirements so that the extract can be formulated in pharmaceutical dosage forms, one of which is a cosmetic preparation in the form of an emulgel. This study aims to see the effect of the addition of EKBD on the physical quality of emulgel preparations and to obtain a stable emulgel product containing standardized EKBD with an accelerated storage process using variations of the gelling agent, namely carbopol 940 and CMC sodium. There are two base variations, namely the Carbopol base with a concentration of 0.25%, 0.5%, 1% with the addition of 1:1 TEA; and CMC sodium base with concentrations of 1%, 2% and 3%. Furthermore, the base and extract are combined until homogeneous. Tests include organoleptic test, pH test, viscosity test, spread ability test, adhesion test and emulsion type test. In organoleptic testing, F1 of both bases showed inhomogeneity and watery texture. In the pH test, both bases F3 did not meet the skin pH requirements. In the viscosity test, F1 on both bases was significantly different before and after storage (p<0.05). In the spread ability test, statistically, all formulas F1, F2 and F3 on both bases showed a value of p>0.05, which means there was no significant difference in the spreadability values before storage and after accelerated storage. In the adhesion test, F3 on the Carbopol base showed a significant difference (p<0.05). In the emulsion type test, both basic formulas had a oil-in-water (O/W) emulsion type. Based on the results of data analysis, the addition of EKBD affected the pH and viscosity of the EKBD emulgel and the formula that met the requirements was formula F2 based on CMC sodium, is a concentration of 2%.

Dengue Hemorrhagic Fever (DHF) is a disease caused by Dengue virus infection. The use of synthetic larvicides causes side effects on human health. Efforts are made to use natural insecticides to minimize contact with dangerous chemical substances. Fragrant pandan leaf plants have secondary metabolite compounds including alkaloids, saponins, flavonoids, tannins and polyphenols, these secondary metabolite compounds can kill larvae. This study aims to determine the effect of variations in the concentration of fragrant pandan leaf extract (P. amaryllifolius Roxb.) on the death of Aedes aegypti Instar III larvae. This type of research is experimental, with a Control Group Post-Test Design. The level of influence of concentration variations is seen from the Lethal Concentration (LC50) and Lethal Time (LT50) values. The data obtained were analyzed using a probit regression test or probit analysis to determine the effect of variations in the concentration of wangi pandan leaf extract (P. amaryllifolius Roxb.) on the death of A. aegypti Instar III larvae. and expressed as Lethal Concentration (LC50) and Lethal Time (LT50) with a confidence level of 95.0%, the probit test obtained the results of LC50-6 hours was 7.770%, LC50-8 hours was 6.516%, LC50-24 hours was 4.409%, LT50-2% is 31,973 hours, LT50-4% is 24,745 hours, and LT50-6% is 16,129 hours, from the parametric tests carried out the results were not normal and homogeneous, then the Kruskal-Wallis test was continued and showed a P-Value 0,000 (<0,05). So it can be said that there is effect of variations in the concentration of fragrant pandan leaf extract (Pandanus amaryllifolius Roxb.) on the mortality of third instar A. aegypti larvae.

Lemon cui also known as fish lemon or kalamansi lemon is known to contain one of them vitamin C which has the potential as an antioxidant so that it can be applied as active ingredient in peel off mask preparations. The aims of this study weretoobtaining the best PVA concentration in the manufacture of peel-off gel masks from lemon cui juice that meet the stability test requirements. physique. The method of making a sample of lemon cui juice is obtained by grinding it using a blender, adding 100 mL of water and then separating the pulp to obtain the juice. The formulation of peel-off gel mask preparations uses variations in the concentration of PVA (Polyvinyl Alcohol), namely F1 (10%), F2 (13%) and F3 (16%). Physical stability tests included organoleptic tests, homogeneity tests, spreadability tests, pH tests, adhesion tests and drying time tests.The results showed that the preparations F1, F2 and F3 showed physical stability based on the results of organoleptic tests, homogeneity tests, spreadability tests, pH tests, adhesion tests and drying time tests before and after accelerated storage conditions. Based on the test results obtained, it can be concluded that the formulation of the Lemon Cui peel-off gel mask preparation from the three concentrations of PVA met the requirements for physical stability. The best PVA concentration is 10% because it has the best dry time.