Abstract

An unusual phospholipid was isolated from a total lipid extract of rabbit lung tissue by chromatography on columns of silica gel H. It accounted for about 0.2 per cent of the total phospholipids. The results of alkaline hydrolysis, periodate oxidation and acetolysis showed that the lipid possessed the structure of a semi-lyso- bis-phosphatidic acid. Its chromatographic properties in four different chromatographic systems supported the proposed structure. It contained mainly palmitic acid (24%), oleic acid (38%) and linoleic acid (20%). The position of the free hydroxyl group in the molecule is discussed.

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