Semen Preservation and Artificial Insemination in Bengal Goat at Field Level

Abstract

Semen ejaculates (n=72) were collected from five Black Bengal Bucks by artificial vagina method. Semen samples were evaluated for volume, sperm cell concentration, motility and morphology. Semen samples were preserved using Tris –Egg yolk-Citrate buffer in liquid state at refrigeration temperature. Rural youths (n=21) were trained on artificial insemination (AI) in goats and preserved semen was used for AI in rural villages of West Bengal and kidding rate was recorded. Black Bengal buck semen was characterized by 0.75 ±0.25 ml volume, 1522 ± 35.30 X 10 6 /ml sperm cell concentration, 85.40 ± 8.20% progressive forward motility and 92.0 ± 1.90% normal morphology. S ignificant (P<0.01) reduction in sperm motility was observed during storage at refrigeration temperature and sperm motility reduced to less than 40% after 96 h of storage. Out of 146 does inseminated with preserved semen, 69 does became pregnant and delivered kids with 47.26% kidding rate. It was concluded that Bengal buck semen can be preserved using Tris –Egg yolk- Citrate buffer at liquid state in refrigeration temperature and can be used for artificial insemination in remote villages over a four days of storage with acceptable kidding rate. Keywords: Goat, semen, preservation, insemination Cite this Article Karunakaran M, Ajoy Mandal, Mohan Mondal et al. Semen Preservation and Artificial Insemination in Bengal Goat at Field Level. Research & Reviews: Journal of Veterinary Science and Technology (RRJoVST) 2015; 4(2): 25–28p.