Abstract
We demonstrate a facile and scalable approach to fabricate and self-assemble the hierarchical hollow microspheres into self-supporting architecture by naturally grown branches. The supporting branches can afford integrated transport channels and significantly improve the kinetic performance and mechanical stability. Meanwhile, the supported hierarchical microspheres acting as functional cell can provide high active sites, multiple response and suitable environment for immobilizing biomolecules. Different enzymes are immobilized for biosensors. The experiments demonstrate that the effective assembly of hierarchical microspheres into large size ordered architecture by self-supporting branches can significantly enhance the biosensing performance.
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