Abstract
Numerous natural compounds are considered as potential therapeutic agents against alcohol-induced liver disease (ALD). Research shows that selenium (Se) has a variety of bioactivities, including liver protecting ability. The present study based on in vitro cell culture models and in vivo mouse models was aimed at examining the contribution of selenomethionine (SeMet)-dominated Se-enriched peanut protein (SePP) to liver protection. SeMet and especially SePP reversed cell viability and cell death, inhibited ethanol induced CYP2E1 activation, decreased reactive oxygen species level, and restored GSH level. Hence, SeMet-dominated SePP alleviates alcohol-induced AML-12 cytotoxicity by suppressing oxidative stress. The p38-dependent mechanism was found to be responsible for SePP-induced Nrf-2 activation. Furthermore, supplementation with SePP and SeMet regulated lipid metabolism and reduced oxidative stress, minimizing liver damage in mice. Selenomethionine-dominated SePP possesses potential therapeutic properties and can be used to treat ALD through the suppression of oxidative stress.
Highlights
The prevalence of liver disease has considerably increased worldwide [1]
Cell death measured by flow cytometry was significantly lower in AML-12 cells treated with 10 μM Se-enriched peanut protein (SePP) 20% (p < 0.001) and SeMet 38% (p < 0.01) compared to cells treated with alcohol (53%) (Figure 2C,D)
Supplementation with SePP and SeMet reduced the level of oxidative stress, and regulated lipid metabolism, reducing the effects of alcohol-induced liver damage in mice
Summary
The prevalence of liver disease has considerably increased worldwide [1]. Various diseases are proven to be associated with oxidative stress, which refers to the negative effects of free radicals in the body [3]. Studies show that ALD development can be exacerbated by reactive oxygen species (ROS) formation and antioxidant activity reduction [2,4]. Plant-based materials, plants, or their active extracts have been determined as potential therapeutic agents of interest for the prevention and treatment of ALD, owing to their low toxicity and multi-target effects [5,6]. Pine nut (Pinus koraiensis) polysaccharides and ginsenosides in ginseng wine have a protective effect on alcohol-induced liver damage [7,8,9]
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