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Abstract
Pseudosolubilized ability of Pseudomonas sp. DG17 on n-alkanes, role of biosurfactants in n-octadecane uptake and trans-membrane transport mechanism of n-octadecane were studied by analyzing amount of pseudosolubilized oil components in water phase, and the fraction of radiolabeled 14C n-octadecane in the broth and cell pellet. GC-MS results showed that pseudosolubilized oil components were mainly C12 to C28 of n-alkanes. In n-octadecane broth, pseudosolubilized n-octadecane could be accumulated as long as pseudosolubilized rate was faster than mineralization rate of substrate, and the maximum concentration of pseudosolubilized n-octadecane achieved to 45.37 mg·L−1. All of these results showed that Pseudomonas sp. DG17 mainly utilized alkanes by directly contacting with pseudosolubilized small oil droplets in the water phase. Analysis of 14C amount in cell pellet revealed that an energy-dependent system mainly controlled the trans-membrane transport of n-octadecane.
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