Abstract
Precipitation of RNA and plasmid DNA in clarified lysate produced from a bacterial culture was studied using three cationic surfactants with a different number of alkyl groups in the hydrophobic tail and cationic head. All the precipitation mixtures contained 0.5 M of NaCl. The results obtained indicated that selective precipitation was achieved when different types of cationic surfactants were used. In this study, trimethyltetradecylammonium bromide (TTAB) appeared to be a promising precipitant for precipitation of supercoiled plasmid DNA.
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