Abstract
Selective detection of Cys and GSH by using one fluorescent probe with two different excitation wavelengths is developed based on a naphthyl-coumarin aldehyde probe. Mechanistic studies indicate that the distinctively different fluorescent responses of the probe toward Cys and GSH at the two excitation wavelengths are due to two different reaction pathways that generate different products. The probe has been used to detect Cys and GSH in HeLa cells by using two emission channels.
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