Abstract
3 Abstract: Staphylococcus spp., is a common bacteria in cattle mastitis. Detection of the low number of bacteria requires a sensitive method. We used Multiplex Polymerase Chain Reaction (M-PCR) assay to detect the genes encoding SEA, SEB, SEC toxins that are specific in Staphylococcus aureus bacteria. The gene of 23S rRNA that is conserved in all Staphylococcus spp., also used as control. Due to the stiffness and low effective enzyme activity such as lyzozyme, proteinase k and mutilysine on bacterial cell wall, it lysed with liquid nitrogen and multiplex PCR was performed after DNA extraction. The obtained results in molecular method were compared with cell culture. The total number of 60 samples of non pasteurized milk was collected in Tehran province restrict from dairy cattle housing and 4 positive samples were detected by PCR and microbial cell culture methods. The accuracy of the test was monitored by using serial dilution (1-l0 ) 6
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
