Abstract
Background Studies using c-kit mutant mast cell (MC)-deficient mice and antibody-mediated depletion of basophils have suggested that both MCs and basophils can contribute to peanut-induced anaphylaxis (PIA) in mice. However, interpretation of data obtained using such approaches to analyze the contributions of individual effector cells to active anaphylaxis is complicated since mice with mutations affecting c-kit structure or expression have several phenotypic abnormalities in addition to their MC deficiency and basophil-depleting antibodies can also react with MCs.
Highlights
Studies using c-kit mutant mast cell (MC)-deficient mice and antibody-mediated depletion of basophils have suggested that both MCs and basophils can contribute to peanut-induced anaphylaxis (PIA) in mice
Inducible and selective ablation of MCs or basophils in non-c-kit mutant mice can significantly reduce PIA, but partial responses to peanut challenge can still be observed in the virtual absence of either cell type, or in mice (Cpa3Cre; Mcl-1fl/fl mice) that virtually lack MCs and have a marked reduction in basophils
The increased levels of neutrophils in KitW-sh/W-sh mice may contribute to the hypothermia induced in these mice in this PIA model
Summary
Selective ablation of mast cells or basophils in mice reduces peanut-induced anaphylaxis. LL Reber1*, T Marichal, K Mukai, A Roers, K Hartmann, H Karasuyama, KC Nadeau, M Tsai, SJ Galli. From Food Allergy and Anaphylaxis Meeting (FAAM 2013) Nice, France. From Food Allergy and Anaphylaxis Meeting (FAAM 2013) Nice, France. 7-9 February 2013
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