Abstract
Due to global warming, high temperature is a significant environmental stress for rice production. Rice (Oryza sativa L.), one of the most crucial cereal crops, is also seriously devastated by Magnaporthe oryzae. Therefore, it is essential to breed new rice cultivars with blast and heat tolerance. Although progress had been made in QTL mapping and RNA-seq analysis in rice in response to blast and heat stresses, there are few reports on simultaneously mining blast-resistant and heat-tolerant genes. In this study, we separately conducted meta-analysis of 839 blast-resistant and 308 heat-tolerant QTLs in rice. Consequently, 7054 genes were identified in 67 blast-resistant meta-QTLs with an average interval of 1.00 Mb. Likewise, 6425 genes were obtained in 40 heat-tolerant meta-QTLs with an average interval of 1.49 Mb. Additionally, using differentially expressed genes (DEGs) in the previous research and GO enrichment analysis, 55 DEGs were co-located on the common regions of 16 blast-resistant and 14 heat-tolerant meta-QTLs. Among, OsChib3H-c, OsJAMyb, Pi-k, OsWAK1, OsMT2b, OsTPS3, OsHI-LOX, OsACLA-2 and OsGS2 were the significant candidate genes to be further investigated. These results could provide the gene resources for rice breeding with excellent resistance to these 2 stresses, and help to understand how plants response to the combination stresses of blast fungus and high temperature.
Highlights
Rice is a significant staple food worldwide that provides more than 20% of the daily caloric needs for at least 50% of the global population [1]
It is essential to mine genes conferring blast resistance and heat tolerance (BR-high temperatures (HT))
By integrating those differentially expressed genes (DEGs), we identified 118 common DEGs (Table S8), which were located on blast-resistant and heat-tolerant meta-QTL regions (Figure 3b)
Summary
Rice is a significant staple food worldwide that provides more than 20% of the daily caloric needs for at least 50% of the global population [1]. To meet the demand of a growing global population, rice yields need a yearly increase of 0.6 to 0.9% [2]. As a consequence of global warming, the combination of pathogens and high temperatures (HT) frequently exists in the cultivation of cereal crops [5,6,7,8]. It is estimated that yearly, the rice blast fungus can destroy enough rice to feed 60 million people [12]. Introgression of the resistant and tolerant genes has been proven to improve resistance and tolerance in existing rice cultivars [15,16,17,18]. It is essential to mine genes conferring blast resistance and heat tolerance (BR-HT)
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