Abstract
Aleurone-free scutella prepared from barley (Hordeum vulgare L. cv Himalaya) grains after 1 day of germination released α-amylase into the incubation medium at a constant rate for at least 2 hours. The release was arrested by a Golgi inhibitor, monensin which caused accumulation of α-amylase inside the scutella instead of the incubation medium. Immunofluorescence labeling showed that α-amylase in the scutella was located in the epithelial cells, no label was found in the parenchyma cells. These results indicate that the scutellar epithelial cells have a true capacity to synthesize and secrete α-amylase.
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