Abstract
A butanol fraction, from the methanolic extract of Opuntia ficus-indica var. saboten, on purification either by preparative TLC or reversed phase HPLC, yielded three chemical components: isorhamnetin 3- O-(6″- O- E-feruloyl)neohesperidoside ( 1), (6 R)-9,10-dihydroxy-4,7-megastigmadien-3-one-9- O-β- d-glucopyranoside ( 2) and (6 S)-9,10-dihydroxy-4,7-megastigmadien-3-one-9- O-β- d-glucopyranoside ( 3) along with 15 known compounds. Structures of compounds ( 1– 3) were elucidated by aid of spectroscopic analyses. The absolute stereochemistry in compounds 2 and 3 was established with the help of CD data analysis and comparison with the literature data. In a DPPH radical scavenging assay, compound 1 showed moderate inhibitory activity (IC 50 = 45.58 μg/ml).
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