Seasons differently impact the structure of mineral weathering bacterial communities in beech and spruce stands

Abstract

Bacterial communities play an essential role in the sustainability of forest ecosystems by releasing from soil minerals the nutritive cations required not only for their own nutrition but also for that of trees. If it is admitted that the nutritional needs of trees vary during seasons, the seasonal dynamics of the mineral weathering bacterial communities colonizing the tree rhizosphere remain unknown. In this study, we characterized the mineral weathering efficacy of bacterial strains, from the rhizosphere and the adjacent bulk soil at four different seasons under two different tree species, the evergreen spruce and the deciduous beech, using a microplate assay that measures the quantity of iron released from biotite. We showed that the functional and taxonomic structures of the mineral weathering bacterial communities varied significantly with the tree species as well as with the season. Notably, the Burkholderia strains from the beech stand appeared more efficient to weather biotite that the one from the spruce stand. The mineral weathering efficacy of the bulk soil isolates did not vary during seasons under the beech stand whereas it was significantly higher for the spring and summer isolates from the spruce stand. The weathering efficacy of the rhizosphere isolates was significantly higher for the autumn isolates compared to the isolates sampled in the other seasons under the beech stand and in summer compared to the other seasons under spruce. These results suggest that seasonal differences do occur in forest soil bacterial communities and that evergreen and deciduous trees do not follow the same dynamic.