Seasonal Fluctuation of Promoters and Inhibitors of Adventitious Root Formation in `Nabali' Olive Shoots

Abstract

Four fractions from known dry weights from buds or leaves of `Nabali' olive shoots—nonacidic ethylacetate (F 1), acidic ether (F 2) and acidic-n-butanol (F 3) were obtained for several sampling dates. A fourth fraction (F 4) from F 1, F 2, and F 3 was established. The four crude fractions were bioassayed for adventitious root formation using mung bean cuttings. In another extraction, fractionation by paper chromatography was performed to identify promoters and inhibitors using the Rf values and mung bean bioassay. The results indicated absence of rooting inhibitors in buds and leaves of `Nabali' olive in mung bean bioassay in all four crude extracts and their fractions located at the various Rf values. However, rooting promoters from buds and leaves were detected by mung bean bioassay in all crude extracts. F 1 contained rooting promoters located at Rf 0.5 and 0.6 throughout the period from 20 Nov. to 2 Apr.; these promoters were found to be highest in buds sampled 23 Feb. and in leaves sampled 4 Dec. and 4 Feb. F 2 from buds and leaves showed neither promoting nor inhibitory activities. F 3 (from leaves or buds) contained promoters of rooting located at Rfs from -.4–0.7 throughout the sampling periods. However, F 3 from buds on 9 Dec., 23 Feb., and 2 Apr.; and from leaves on 4 Feb. gave significantly highest root-promoting activity. F 4 from buds and leaves showed root-promoting effects from November to April. The promoting effect of F 4 was greatest in buds and leaves sampled 9 Dec. and 4 Feb., respectively.