Seasonal Dynamics of Soil Bacterial Community under Long-Term Abandoned Cropland in Boreal Climate

Abstract

The collapse of collective farming in Russia after 1990 led to the abandonment of 23% of the agricultural area. Microbial biomass is a transit pool between fresh and soil organic matter; therefore, structural changes in soil microbial community determine the carbon cycle processes caused by self-restoration of arable lands after abandonment. Here, we assessed the influence of monthly changes in moisture and temperature on the bacterial community structure and abundance in Retisols under long-term abandoned cropland. Two periods with pronounced differences in bacterial properties were revealed: the growing period from March to September and the dormant period from October to February. The growing period was characterized by higher bacterial abundance and diversity compared to the dormant period. The relative abundances of the bacterial community dominants (Alpha-, Gamma- and Deltaproteobacteria, subgroup 6 of phylum Acidobacteria) did not change significantly over the year, either in total or active communities. The relative abundances of Bacteroidetes and Verrucomicrobia increased in the growing period, whereas Actinobacteria and Chloroflexi were more abundant in the dormant period. The microbial gene abundances positively correlated with soil and air temperature, but not with soil moisture. Thus, the seasonal dynamics of soil microbial communities are closely related to soil temperature and should be considered when assessing carbon cycles in abandoned lands.