Search for the new anti-inflammatory agents based on glycosylated polypeptide complex extracted from sea urchins Strongylocentrotus droebachiensis

Abstract

We performed the study of the anti-inflammatory activity of glycosylated polypeptide complex (GPC) extracted from sea urchins Strongylocentrotus droebachiensis in the model of acute bronchitis induced by endotracheal administration of 1% formalin solution in male Wistar rats. Animals of experimental groups were inhaled by GPC solution at 25, 50 and 100 mcg/kg, control animals – by the reference drug ambroxol at 3.6 mg/kg once daily during 7 days starting from the day of pathology induction. 24 hours after pathology induction GPC at 100 mcg/kg reduced the total leukocyte count in the bronchoalveolar lavage fluid by 34%, after 48 hours – by 61% compared with control animal group (р ≤ 0.05). If the normal number of goblet cells per 1 mm bronchial mucosa was 33.7 ± 1.7 and after formalin exposure – 67.5 ± 1.8, then after a course administration of polypeptide complex (7 days) at 100 mcg/kg – 55.7 ± 2.7. By the results of morphology and histology study we determined that mononuclear infiltration of bronchial submucosa layer by lymphocytes and macrophages, neutrophil leukocytes of mucosa and submucosa was significantly less than in control animal group (р < 0.05) affected by formalin without any pharmacological protection. Upon the results of the study, we determined the anti-inflammatory activity of glycosylated polypeptide complex at 25, 50 and 100 mcg/kg, which was not inferior to the similar activity of ambroxol (3.6 mg/kg).