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Abstract
Abstract Caffeine is a compound contained in coffee and is a trigger for digestive disorders such as ulcers. Hypothenemus hampei, a coffee berry borer, can adapt to the caffeine content in coffee because symbiotic bacteria can digest caffeine in the gut of the insect. This study aims to isolate and characterise symbiotic bacteria associated with the gut of H. hampei. Symbiotic bacteria in the gut of the insect were isolated and tested by growing in various artificial growth media, which include caffeine test, pathogenicity test, human gastric acid resistance test, and salt osmotic resistance test. As many as 50 bacteria isolates were obtained, but only eight isolates could grow on the caffeine test. Furthermore, the pathogenicity test showed that 2 bacterial isolates were harmful to humans and 6 were safe for humans. All isolates showed positive results on the human gastric acid and salt osmotic resistance tests. Three genera of bacteria were successfully identified based on morphology and physiology characters, i.e., Chromobacterium sp., Micrococcus sp., and Enterobacter sp. Further evaluation and experiments are needed to explore the potential of symbiotic bacteria for beneficial functions.
Published Version
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